利用超临界流体萃取技术从废咖啡渣油中生产聚羟基烷酸酯。
Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology.
机构信息
REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
CEER - Biosystems Engineering Center, Institute of Agronomy, University of Lisbon, 1349-017 Lisboa, Portugal.
出版信息
Bioresour Technol. 2014 Apr;157:360-3. doi: 10.1016/j.biortech.2014.02.013. Epub 2014 Feb 14.
Spent coffee grounds (SCG) oil was obtained by supercritical carbon dioxide (scCO2) extraction in a pilot plant apparatus, with an oil extraction yield of 90% at a 35kgkg(-1) CO2/SCG ratio. Cupriavidus necator DSM 428 was cultivated in 2L bioreactor using extracted SCG oil as sole carbon source for production of polyhydroxyalkanoates. The culture reached a cell dry weight of 16.7gL(-1) with a polymer content of 78.4% (w/w). The volumetric polymer productivity and oil yield were 4.7gL(-1)day(-1) and 0.77gg(-1), respectively. The polymer produced was a homopolymer of 3-hydroxybutyrate with an average molecular weight of 2.34×10(5) and a polydispersity index of 1.2. The polymer exhibited brittle behaviour, with very low elongation at break (1.3%), tensile strength at break of 16MPa and Young's Modulus of 1.0GPa. Results show that SCG can be a bioresource for polyhydroxyalkanoates production with interesting properties.
废咖啡渣（SCG）油是通过在中试工厂设备中用超临界二氧化碳（scCO2）提取得到的，在 35kgkg(-1) CO2/SCG 比下，油提取收率为 90%。在 2L 生物反应器中，用提取的 SCG 油作为唯一碳源培养 Cupriavidus necator DSM 428 生产聚羟基烷酸酯。培养物达到了 16.7gL(-1)的细胞干重，聚合物含量为 78.4%（w/w）。体积聚合物生产率和油收率分别为 4.7gL(-1)day(-1)和 0.77gg(-1)。所生产的聚合物是 3-羟基丁酸的均聚物，重均分子量为 2.34×10(5)，多分散指数为 1.2。该聚合物表现出脆性行为，断裂伸长率极低（1.3%），断裂拉伸强度为 16MPa，杨氏模量为 1.0GPa。结果表明，SCG 可以作为聚羟基烷酸酯生产的生物资源，具有有趣的性能。
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