School of Civil Engineering, Wuhan University, Wuhan 430072, China.
School of Civil Engineering, Wuhan University, Wuhan 430072, China.
Bioresour Technol. 2014 Apr;158:156-60. doi: 10.1016/j.biortech.2014.02.025. Epub 2014 Feb 15.
A continuous-upflow submerged biofilter packed with ceramsite was constructed for nitrate removal under an aerobic atmosphere. Pseudomonas stutzeri X31, an aerobic denitrifier isolate, was added to the bioreactor as an inoculum. The influent NO3(-)-N concentrations were 63.0-73.8 mg L(-1). The best results were achieved when dissolved oxygen level was 4.6 mg L(-1) and C/N ratio was 4.5. The maximum removal efficiencies of carbon oxygen demand (COD) and NO3(-)-N were 94.04% and 98.48%, respectively at 30°C, when the hydraulic load was 0.75 m h(-1). The top section of the bioreactor possessed less biofilm but higher COD and NO3(-)-N removal rates than the bottom section. Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technique combined with electron microscopic examination indicated P. stutzeri X31 and Paracoccus versutus were the most dominant bacteria. Amoeba sp., Vorticella sp., Philodina sp., and Stephanodiscus sp. were also found in the bioreactor.
采用连续上流淹没式生物滤池，以陶粒作为填料，在有氧条件下进行硝酸盐去除。将好氧反硝化菌 Pseudomonas stutzeri X31 作为接种物添加到生物反应器中。进水 NO3(-)-N 浓度为 63.0-73.8 mg/L。当溶解氧水平为 4.6 mg/L，C/N 比为 4.5 时，效果最佳。在 30°C 时，水力负荷为 0.75 m h(-1)，COD 和 NO3(-)-N 的最大去除效率分别为 94.04%和 98.48%。生物反应器的顶部区域生物膜较少，但 COD 和 NO3(-)-N 的去除率高于底部区域。聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）技术结合电子显微镜检查表明，P. stutzeri X31 和 Paracoccus versutus 是最主要的细菌。在生物反应器中还发现了变形虫、钟虫、膜盘虫和舟形藻。
