Zhang Cong-En, Xiong Yin, Dong Qin, Gao Dan, Zhang Le-Le, Ma Li-Na, Peng Cheng, Dong Xiao-Ping, Yan Dan
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China; Military Institute of Chinese Materia Medica, 302th Military Hospital of China, Beijing, China.
J Sep Sci. 2014 May;37(9-10):1141-7. doi: 10.1002/jssc.201301159. Epub 2014 Apr 10.
Radix isatidis is a famous anti-influenza virus herbal medicine traditionally taken as a water decoction. However, the chemical fingerprint analysis of Radix isatidis is dominantly based on RPLC, from which it is difficult to obtain fingerprint information of hydrophilic compounds. Here, we developed the separation of Radix isatidis by RPLC and hydrophilic interaction chromatography, comparing the traditional RPLC fingerprint with the hydrophilic interaction chromatography fingerprint. Besides, an anti-viral assay of Radix isatidis was conducted to evaluate its efficacy. The fingerprint-efficacy relationships between the fingerprints and the anti-viral activity were further investigated with principal component regression analysis. The results showed that the anti-viral activity correlated better with the hydrophilic interaction chromatography fingerprint than with the RPLC fingerprint. This study indicates that hydrophilic interaction chromatography could not only be a complementary method to increase the fingerprint coverage of conventional RPLC fingerprint, but also can better represent the efficacy and quality of Radix isatidis.
板蓝根是一种著名的抗流感病毒草药，传统上采用水煎剂服用。然而，板蓝根的化学指纹图谱分析主要基于反相液相色谱法（RPLC），从中难以获得亲水性化合物的指纹信息。在此，我们开发了通过RPLC和亲水相互作用色谱法对板蓝根进行分离的方法，将传统的RPLC指纹图谱与亲水相互作用色谱指纹图谱进行比较。此外，对板蓝根进行了抗病毒试验以评估其疗效。采用主成分回归分析进一步研究了指纹图谱与抗病毒活性之间的指纹-疗效关系。结果表明，抗病毒活性与亲水相互作用色谱指纹图谱的相关性优于RPLC指纹图谱。本研究表明，亲水相互作用色谱法不仅可以作为一种补充方法来增加传统RPLC指纹图谱的指纹覆盖范围，而且能够更好地反映板蓝根的疗效和质量。
