Wernecke Kerstin, Lamprecht Ingolf, Jöhren Olaf, Lehnert Hendrik, Schulz Carla
Department of Internal Medicine I, University of Luebeck, Ratzeburger Allee 160, 23538, Luebeck, Germany.
Obesity (Silver Spring). 2014 Jul;22(7):1662-8. doi: 10.1002/oby.20736. Epub 2014 Mar 25.
Energy homeostasis results from a balance of food intake and energy expenditure, accomplished by the interaction of peripheral and central nervous signals. The recently discovered adipokine nesfatin-1 is involved in the central control of food intake, but whether it also participates in the regulation of thermogenesis is unknown.
Nesfatin-1 was administered intracerebroventricularly to freely moving, male Wistar rats and direct calorimetry was performed to assess its effects on thermogenesis. Furthermore, food intake was measured and hypothalamic and N. tractus solitarius (NTS) neuropeptide expression was determined by quantitative real-time polymerace chain reaction. Leptin, which is involved in both the regulation of food intake and thermogenesis, was used as positive control.
For the first time it was shown that central nervous administration of nesfatin-1 profoundly increases thermogenesis in rats to a similar extent as leptin and the role of both peptides in the control of food intake was confirmed. Nesfatin-1 significantly downregulated neuropeptide Y (NPY) mRNA expression in both hypothalamus and NTS.
The results strongly support the prominent role of nesfatin-1 for both energy expenditure and food intake and NPY neurons appear to be involved in this effect.
能量平衡源于食物摄入与能量消耗的平衡，这是由外周和中枢神经信号的相互作用实现的。最近发现的脂肪因子nesfatin-1参与食物摄入的中枢控制，但它是否也参与产热调节尚不清楚。
将nesfatin-1经脑室注射给予自由活动的雄性Wistar大鼠，并采用直接测热法评估其对产热的影响。此外，测量食物摄入量，并通过定量实时聚合酶链反应测定下丘脑和孤束核（NTS）神经肽的表达。将参与食物摄入和产热调节的瘦素用作阳性对照。
首次表明，向大鼠中枢神经系统给药nesfatin-1可显著增加产热，其程度与瘦素相似，并且证实了这两种肽在控制食物摄入中的作用。Nesfatin-1显著下调下丘脑和NTS中神经肽Y（NPY）的mRNA表达。
结果有力地支持了nesfatin-1在能量消耗和食物摄入方面的重要作用，并且NPY神经元似乎参与了这一效应。
