Saito Reiko, Sonoda Satomi, Ueno Hiromichi, Motojima Yasuhito, Yoshimura Mitsuhiro, Maruyama Takashi, Hashimoto Hirofumi, Tanaka Kentaro, Yamamoto Yukiyo, Kusuhara Koichi, Ueta Yoichi
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka, 807-8555, Japan; Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka 807-8555, Japan.
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka, 807-8555, Japan.
Neurosci Lett. 2017 Aug 10;655:54-60. doi: 10.1016/j.neulet.2017.06.049. Epub 2017 Jul 3.
Peripheral anorectic hormones, such as peptide YY (PYY) and oxytocin (OXT), suppress food intake. A newly identified anorectic neuropeptide, nesfatin-1, is synthesized in both peripheral tissue and the central nervous system, particularly by various nuclei in the hypothalamus and brainstem. Here, we examined the effects of intraperitoneal (ip) administration of PYY, OXT, and OXT analog, on nesfatin-1-immunoreactive (ir) neurons in the rat hypothalamus and brainstem, using Fos double fluorescence-immunohistochemistry. The ip administration of OXT and OXT analog significantly increased the number of nesfatin-1-ir neurons expressing Fos-ir in the paraventricular nucleus, the arcuate nucleus, and the nucleus tractus solitarius, but not in the supraoptic nucleus, the lateral hypothalamic area, and the area postrema. No differences in the percentage of nesfatin-1-ir neurons expressing Fos in the nuclei of the hypothalamus and brainstem were observed, between rats treated with vehicle or those treated with PYY. The decreased food intake, induced by OXT and OXT analog, was attenuated significantly by pretreatment with intracerebroventricular administration of antisense nesfatin-1. These results suggested that nesfatin-1-expressing neurons in the hypothalamus and brainstem may play a role in sensing the peripheral level of OXT and its suppression of feeding in rats.
外周食欲调节激素，如肽YY（PYY）和催产素（OXT），可抑制食物摄入。一种新发现的食欲调节神经肽——内脂素-1，在外周组织和中枢神经系统中均有合成，尤其是在下丘脑和脑干的各个核团中。在此，我们使用Fos双荧光免疫组织化学方法，研究了腹腔注射PYY、OXT和OXT类似物对大鼠下丘脑和脑干中内脂素-1免疫反应性（ir）神经元的影响。腹腔注射OXT和OXT类似物显著增加了室旁核、弓状核和孤束核中表达Fos-ir的内脂素-1-ir神经元数量，但在视上核、下丘脑外侧区和最后区中未增加。在用载体处理的大鼠和用PYY处理的大鼠之间，未观察到下丘脑和脑干核团中表达Fos的内脂素-1-ir神经元百分比的差异。脑室注射反义内脂素-1预处理可显著减弱由OXT和OXT类似物诱导的食物摄入量减少。这些结果表明，下丘脑和脑干中表达内脂素-1的神经元可能在感知大鼠外周OXT水平及其对进食的抑制作用中发挥作用。
