Sharman M J, Bacci B, Whittem T, Mansfield C S
Translational Research and Animal Clinical Trial Study (TRACTS) Group, the Faculty of Veterinary Science, The University of Melbourne, Melbourne, Vic., Australia.
J Vet Intern Med. 2014 May-Jun;28(3):799-808. doi: 10.1111/jvim.12332. Epub 2014 Mar 5.
Confocal endomicroscopy (CEM) is an endoscopic technology permitting in vivo cellular and subcellular imaging. CEM aids real-time clinical assessment and diagnosis of various gastrointestinal diseases in people. CEM allows in vivo characterization of small intestinal mucosal morphology in dogs.
To determine the feasibility of CEM to evaluate gastric mucosal morphology in dogs and to characterize the appearance in healthy dogs.
Fourteen clinically healthy research colony dogs.
Experimental study. Under general anesthesia, dogs underwent standard endoscopic evaluation and CEM of the gastric mucosa. In the initial 6 dogs, fluorescent contrast was provided with the fluorophore acriflavine (0.05% solution), applied topically. Subsequently, 8 dogs were assessed using a combination of fluorescein (10% solution, 15 mg/kg IV), followed by acriflavine administered topically. For each fluorophore, a minimum of 5 sites were assessed.
Confocal endomicroscopy provided high quality in vivo histologically equivalent images of the gastric mucosa, but reduced flexibility of the endoscope tip limited imaging of the cranial stomach in some dogs. Intravenous administration of fluorescein allowed assessment of cellular cytoplasmic and microvasculature features. Topical application of acriflavine preferentially stained cellular nucleic acids, allowing additional evaluation of nuclear morphology. Identification of Helicobacter-like organisms was possible in 13 dogs.
Confocal endomicroscopy provides in vivo images allowing assessment of gastric mucosal morphology during endoscopy, potentially permitting real-time diagnosis of gastrointestinal disease.
共聚焦内镜显微镜检查（CEM）是一种允许进行体内细胞和亚细胞成像的内镜技术。CEM有助于对人类各种胃肠道疾病进行实时临床评估和诊断。CEM可对犬小肠黏膜形态进行体内特征描述。
确定CEM评估犬胃黏膜形态的可行性，并描述健康犬的表现。
14只临床健康的实验犬。
实验研究。在全身麻醉下，对犬进行标准内镜评估和胃黏膜的CEM检查。最初的6只犬，通过局部应用荧光团吖啶黄（0.05%溶液）提供荧光对比。随后，对8只犬先静脉注射荧光素（10%溶液，15mg/kg），然后局部应用吖啶黄进行评估。对于每种荧光团，至少评估5个部位。
共聚焦内镜显微镜检查提供了高质量的胃黏膜体内组织学等效图像，但内镜尖端灵活性降低限制了部分犬胃近端的成像。静脉注射荧光素可评估细胞胞质和微血管特征。局部应用吖啶黄优先对细胞核酸进行染色，从而可进一步评估核形态。13只犬中有可能识别出类幽门螺杆菌生物。
共聚焦内镜显微镜检查提供的体内图像可在内镜检查期间评估胃黏膜形态，有可能实现胃肠道疾病的实时诊断。
