来自假单胞菌属菌株ADP的脲基甲酸酯水解酶酰胺酶结构域的结晶及初步X射线衍射分析。
Crystallization and preliminary X-ray diffraction analysis of the amidase domain of allophanate hydrolase from Pseudomonas sp. strain ADP.
作者信息
Balotra Sahil, Newman Janet, French Nigel G, Briggs Lyndall J, Peat Thomas S, Scott Colin
机构信息
Ecosystem Sciences, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
Materials, Science and Engineering, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.
出版信息
Acta Crystallogr F Struct Biol Commun. 2014 Mar;70(Pt 3):310-5. doi: 10.1107/S2053230X13034705. Epub 2014 Feb 19.
The allophanate hydrolase from Pseudomonas sp. strain ADP was expressed and purified, and a tryptic digest fragment was subsequently identified, expressed and purified. This 50 kDa construct retained amidase activity and was crystallized. The crystals diffracted to 2.5 Å resolution and adopted space group P21, with unit-cell parameters a = 82.4, b = 179.2, c = 112.6 Å, β = 106.6°.
来自假单胞菌属菌株ADP的脲基甲酸酯水解酶被表达并纯化，随后鉴定、表达并纯化了一个胰蛋白酶消化片段。这个50 kDa的构建体保留了酰胺酶活性并被结晶。晶体衍射分辨率达到2.5 Å，属于P21空间群，晶胞参数为a = 82.4、b = 179.2、c = 112.6 Å，β = 106.6°。
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