Fülöp András, Szijártó Attila, Harsányi László, Budai András, Pekli Damján, Korsós Diána, Horváth Ildikó, Kovács Noémi, Karlinger Kinga, Máthé Domokos, Szigeti Krisztián
1st Department of Surgery, Semmelweis University, Budapest, Hungary.
Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
PLoS One. 2014 Mar 5;9(3):e90760. doi: 10.1371/journal.pone.0090760. eCollection 2014.
In the early recognition of portal vein ligation (PVL) induced tumor progression, positron emission tomography and magnetic resonance imaging (PET/MRI) could improve diagnostic accuracy of conventionally used methods. It is unknown how PVL affects metabolic patterns of tumor free hepatic tissues. The aim of this preliminary study is to evaluate the effect of PVL on glucose metabolism, using PET/MRI imaging in healthy rat liver.
Male Wistar rats (n=30) underwent PVL. 2-deoxy-2-(18F)fluoro-D-glucose (FDG) PET/MRI imaging (nanoScan PET/MRI) and morphological/histological examination were performed before (Day 0) and 1, 2, 3, and 7 days after PVL. Dynamic PET data were collected and the standardized uptake values (SUV) for ligated and non-ligated liver lobes were calculated in relation to cardiac left ventricle (SUVVOI/SUVCLV) and mean liver SUV (SUVVOI/SUVLiver).
PVL induced atrophy of ligated lobes, while non-ligated liver tissue showed compensatory hypertrophy. Dynamic PET scan revealed altered FDG kinetics in both ligated and non-ligated liver lobes. SUVVOI/SUVCLV significantly increased in both groups of lobes, with a maximal value at the 2nd postoperative day and returned near to the baseline 7 days after the ligation. After PVL, ligated liver lobes showed significantly higher tracer uptake compared to the non-ligated lobes (significantly higher SUVVOI/SUVLiver values were observed at postoperative day 1, 2 and 3). The homogenous tracer biodistribution observed before PVL reappeared by 7th postoperative day.
The observed alterations in FDG uptake dynamics should be taken into account during the assessment of PET data until the PVL induced atrophic and regenerative processes are completed.
在门静脉结扎（PVL）诱导的肿瘤进展的早期识别中，正电子发射断层扫描和磁共振成像（PET/MRI）可提高传统方法的诊断准确性。目前尚不清楚PVL如何影响无肿瘤肝组织的代谢模式。本初步研究的目的是使用PET/MRI成像评估PVL对健康大鼠肝脏葡萄糖代谢的影响。
雄性Wistar大鼠（n = 30）接受PVL手术。在PVL术前（第0天）以及术后1、2、3和7天进行2-脱氧-2-（18F）氟-D-葡萄糖（FDG）PET/MRI成像（nanoScan PET/MRI）以及形态学/组织学检查。收集动态PET数据，并计算结扎和未结扎肝叶相对于心脏左心室的标准化摄取值（SUV）（SUVVOI/SUVCLV）以及肝脏平均SUV（SUVVOI/SUVLiver）。
PVL导致结扎叶萎缩，而未结扎的肝组织出现代偿性肥大。动态PET扫描显示结扎和未结扎肝叶的FDG动力学均发生改变。两组肝叶的SUVVOI/SUVCLV均显著增加，在术后第2天达到最大值，并在结扎后7天恢复至接近基线水平。PVL术后，结扎肝叶的示踪剂摄取显著高于未结扎肝叶（在术后第1、2和3天观察到显著更高的SUVVOI/SUVLiver值）。PVL术前观察到的示踪剂均匀生物分布在术后第7天再次出现。
在PVL诱导的萎缩和再生过程完成之前，在评估PET数据时应考虑到观察到的FDG摄取动力学变化。
