Xu Rui, Yuan Yu-Feng, Ayav Ahmet, Jiang Chong-Qing, Bresler Laurent, Liu Zhi-Su, Tran Nguyen
General Surgery Department, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China ; School of Surgery, Faculty of Medicine-UHP, University of Nancy, Nancy 54501, France.
School of Surgery, Faculty of Medicine-UHP, University of Nancy, Nancy 54501, France.
Exp Ther Med. 2014 May;7(5):1089-1094. doi: 10.3892/etm.2014.1607. Epub 2014 Mar 6.
The aim of the present study was to investigate the effect of portal vein ligation (PVL) on the tumor growth rate and liver regeneration in rat cirrhotic liver lobes. A total of 45 male Wistar rats were randomly divided into PVL, hepatic tumor (HT) and HT + PVL groups (n=15 per group). Liver regeneration and tumor growth in ligated and non-ligated lobes were evaluated prior to and following PVL. In addition, serum alanine transaminase, total bilirubin levels and liver tissue samples were evaluated. The results indicated that PVL induced apparent hypertrophy in normal and HT rats. However, the ratio of non-ligated lobes to total liver weight or body weight in the HT + PVL group was significantly lower when compared with the PVL group (P<0.05). Compared with the HT group, the tumor growth rate in the ligated lobes of the HT + PVL group significantly increased (P<0.05). However, tumor growth in the non-ligated lobes exhibited no statistically significant difference between the HT and HT + PVL groups. In addition, Knodell scores indicated that fibrosis was more apparent in the non-ligated lobes of the HT + PVL group when compared with the HT group (P<0.05). Therefore, tumor growth was accelerated in ligated lobes following PVL, but not in non-ligated lobes. PVL also induced liver regeneration in cirrhotic liver lobes with lower efficiency than that in the non-cirrhotic lobes. However, hypertrophy in the contralateral cirrhotic lobes appeared to be non-functional.
本研究的目的是探讨门静脉结扎（PVL）对大鼠肝硬化肝叶肿瘤生长速率和肝再生的影响。总共45只雄性Wistar大鼠被随机分为PVL组、肝肿瘤（HT）组和HT + PVL组（每组n = 15）。在PVL之前和之后评估结扎和未结扎肝叶的肝再生和肿瘤生长情况。此外，还评估了血清丙氨酸转氨酶、总胆红素水平以及肝组织样本。结果表明，PVL诱导正常大鼠和HT大鼠出现明显的肥大。然而，与PVL组相比，HT + PVL组中未结扎肝叶与肝脏总重量或体重的比值显著降低（P<0.05）。与HT组相比，HT + PVL组结扎肝叶中的肿瘤生长速率显著增加（P<0.05）。然而，HT组和HT + PVL组之间未结扎肝叶中的肿瘤生长没有统计学上的显著差异。此外，Knodell评分表明，与HT组相比，HT + PVL组未结扎肝叶中的纤维化更明显（P<0.05）。因此，PVL后结扎肝叶中的肿瘤生长加速，但未结扎肝叶中未加速。PVL还诱导肝硬化肝叶的肝再生，但其效率低于非肝硬化肝叶。然而，对侧肝硬化肝叶中的肥大似乎没有功能。
