Department of Surgery, University of Cincinnati, Cincinnati, OH 45267-0558, United States.
World J Gastroenterol. 2010 Aug 14;16(30):3816-26. doi: 10.3748/wjg.v16.i30.3816.
To investigate the mechanisms of liver growth and atrophy after portal vein ligation (PVL) and its effects on tumor growth.
Mice were subjected to PVL, partial hepatectomy, or sham surgery. The morphological alterations, activation of transcription factors, and expression of cytokines and growth factors involved in liver regeneration were evaluated. In a separate set of experiments, murine colorectal carcinoma cells were injected via the portal vein and the effect of each operation on liver tumor growth was studied.
Liver regeneration after PVL and partial hepatectomy were very similar. In ligated lobes, various cytokines, transcription factors and regulatory factors were significantly upregulated compared to non-ligated lobes after PVL. Atrophy in ligated lobes was a result of early necrosis followed by later apoptosis. Tumor growth was significantly accelerated in ligated compared to non-ligated lobes.
Tumor growth was accelerated in ligated liver lobes and appeared to be a result of increased growth factor expression.
探讨门静脉结扎（PVL）后肝生长和萎缩的机制及其对肿瘤生长的影响。
对小鼠进行 PVL、部分肝切除术或假手术。评估涉及肝再生的形态改变、转录因子激活以及细胞因子和生长因子表达。在一组单独的实验中，通过门静脉注射鼠结直肠癌细胞，并研究每种手术对肝肿瘤生长的影响。
PVL 后肝再生和部分肝切除非常相似。与 PVL 后的非结扎叶相比，结扎叶中的各种细胞因子、转录因子和调节因子显著上调。结扎叶的萎缩是早期坏死后继发凋亡的结果。与非结扎叶相比，结扎叶中的肿瘤生长明显加快。
结扎肝叶中的肿瘤生长加快，似乎是生长因子表达增加的结果。
