Interdisciplinary Division of Biomedical Engineering, the Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P. R. China.
Small. 2014 Jun 25;10(12):2390-7. doi: 10.1002/smll.201303766. Epub 2014 Mar 5.
Avian influenza viruses (AIV) with good adaptation and various mutations have threatened both human and animals' health. The H7 subtypes have the potential to cause pandemic threats to human health due to the highly pathogenic characteristics. Therefore, it is quite urgent to develop a novel biosensor for rapid and sensitive detection of H7 subtypes. In this work, a biosensor based on luminescence resonance energy transfer (LRET) from BaGdF5:Yb/Er upconversion nanoparticles (UCNPs) to gold nanoparticles (AuNPs) has been developed for rapid and sensitive H7 subtypes detection. The amino modified capture oligonucleotide probes are covalently linked to poly(ethylenimine) (PEI) modified BaGdF5:Yb/Er UCNPs. The thiol modified oligonucleotides with H7 hemagglutinin gene sequence are conjugated to surfaces of AuNPs. The hybridization process between complementary strands of H7 Hemagglutinin gene and its probe brings the energy donor and acceptor into close proximity, leading to the quenching of fluorescence of UCNPs. A linear response is obtained ranging from 10 pm to 10 nm and the limit of detection (LOD) is around 7 pm with detection time around 2 hours. This biosensor is expected to be a valuable diagnostic tool for rapid and sensitive detection of AIV.
禽流感病毒（AIV）具有良好的适应性和多种突变，威胁着人类和动物的健康。由于其高致病性特征，H7 亚型有潜力对人类健康造成大流行威胁。因此，开发一种新型的生物传感器来快速灵敏地检测 H7 亚型是非常紧迫的。在这项工作中，我们基于上转换纳米粒子（UCNPs）到金纳米粒子（AuNPs）的发光共振能量转移（LRET）开发了一种生物传感器，用于快速灵敏地检测 H7 亚型。经氨基修饰的捕获寡核苷酸探针通过共价键连接到聚（乙二胺）（PEI）修饰的 BaGdF5:Yb/Er UCNPs 上。具有 H7 血凝素基因序列的巯基修饰的寡核苷酸连接到 AuNPs 的表面。H7 血凝素基因与其探针的互补链之间的杂交过程使供体和受体接近，导致 UCNPs 的荧光猝灭。从 10 pm 到 10 nm 范围内获得线性响应，检测时间约为 2 小时，检测限（LOD）约为 7 pm。这种生物传感器有望成为快速灵敏检测 AIV 的有价值的诊断工具。
