Ma Yingjin, Song Menglin, Li Lihua, Lao Xinyue, Wong Man-Chung, Hao Jianhua
Department of Applied Physics The Hong Kong Polytechnic University Hong Kong China.
Exploration (Beijing). 2022 Oct 12;2(6):20210216. doi: 10.1002/EXP.20210216. eCollection 2022 Dec.
Various infectious viruses have been posing a major threat to global public health, especially SARS-CoV-2, which has already claimed more than six million lives up to now. Tremendous efforts have been made to develop effective techniques for rapid and reliable pathogen detection. The unique characteristics of upconversion nanoparticles (UCNPs) pose numerous advantages when employed in biosensors, and they are a promising candidate for virus detection. Herein, this Review will discuss the recent advancement in the UCNP-based biosensors for virus and biomarkers detection. We summarize four basic principles that guide the design of UCNP-based biosensors, which are utilized with luminescent or electric responses as output signals. These strategies under fundamental mechanisms facilitate the enhancement of the sensitivity of UCNP-based biosensors. Moreover, a detailed discussion and benefits of applying UCNP in various virus bioassays will be presented. We will also address some obstacles in these detection techniques and suggest routes for progress in the field. These progressions will undoubtedly pose UCNP-based biosensors in a prominent position for providing a convenient, alternative approach to virus detection.
各种传染性病毒一直对全球公共卫生构成重大威胁，尤其是严重急性呼吸综合征冠状病毒2（SARS-CoV-2），截至目前，它已夺走了600多万人的生命。人们已付出巨大努力来开发快速、可靠的病原体检测有效技术。上转换纳米颗粒（UCNP）的独特特性在用于生物传感器时具有诸多优势，是病毒检测的一个有前途的候选者。在此，本综述将讨论基于UCNP的用于病毒和生物标志物检测的生物传感器的最新进展。我们总结了指导基于UCNP的生物传感器设计的四个基本原理，这些原理以发光或电响应作为输出信号。这些基于基本机制的策略有助于提高基于UCNP的生物传感器的灵敏度。此外，还将详细讨论在各种病毒生物测定中应用UCNP的情况及其益处。我们还将探讨这些检测技术中的一些障碍，并提出该领域的进展途径。这些进展无疑将使基于UCNP的生物传感器在提供一种方便的病毒检测替代方法方面占据突出地位。
