Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
J Fluoresc. 2022 Jul;32(4):1337-1346. doi: 10.1007/s10895-022-02907-4. Epub 2022 Apr 2.
In this study, a sensitive fluorimetric method is proposed for the determination of piroxicam using nitrogen graphene quantum dots (N-GQDs) and gold nanoparticles coated with phenylalanine. The fluorescence emission of N-GQDs at 440 nm decreases with the increase of gold nanoparticles coated with phenylalanine. However, the addition of piroxicam causes the release of gold nanoparticles from the surface of quantum dots followed by the retrieval of the fluorescence emission of N-GQDs. Under the optimum conditions, the calibration graph was linear in the concentration range of 2.0-35.0 nmol L for piroxicam with a limit of detection of 0.11 nmol L. The developed method was successfully applied for the determination of piroxicam in urine and serum samples.
本研究提出了一种使用氮掺杂石墨烯量子点（N-GQDs）和苯丙氨酸修饰的金纳米粒子测定吡罗昔康的灵敏荧光法。N-GQDs 在 440nm 处的荧光发射强度随着苯丙氨酸修饰的金纳米粒子的增加而降低。然而，加入吡罗昔康会导致金纳米粒子从量子点表面释放出来，随后恢复 N-GQDs 的荧光发射。在最佳条件下，吡罗昔康的浓度范围在 2.0-35.0nmol L 内呈线性，检出限为 0.11nmol L。该方法成功应用于尿液和血清样品中吡罗昔康的测定。
