基质金属蛋白酶-2/基质金属蛋白酶-9启动子多态性与缺血性中风之间的相关性。
Correlations between MMP-2/MMP-9 promoter polymorphisms and ischemic stroke.
作者信息
Nie Shu-Wei, Wang Xiao-Feng, Tang Zong-Chun
机构信息
Department of Emergency, The Fourth Peoples Hospital in Shaanxi Province Xi'an 710043, China.
Department of Neurosurgery, The Third Hospital of Chinese Peoples Liberation Army Baoji 721004, China.
出版信息
Int J Clin Exp Med. 2014 Feb 15;7(2):400-4. eCollection 2014.
OBJECTIVE
Ischemic stroke (IS) results from interrupted blood flow to the brain and can be caused by formation of atherosclerotic plaques in blood vessels. Previous studies have suggested a contribution of matrix metalloproteinases (MMP) to plaque formation and stroke risk. Here, we explored associations between two MMPs, MMP-2 and MMP-9, and IS.
METHODS
There were 396 IS patients and 400 control individuals involved in this study. The MMP-2 -1306C/T and -735C/T polymorphisms and MMP-9 -1562C/T polymorphism were analyzed by restriction fragment length polymorphism (RFLP) from patient blood samples, and the relationships to IS were analyzed by logistic regression.
RESULTS
Genotype and allele frequencies of MMP-2 -1306C/T did not differ statistically (P>0.05), but those of MMP-2 -735C/T and MMP-9 1562C/T did differ between IS and control samples (P<0.05). While MMP-2 -1306C/T polymorphism was not associated with increased risk of IS, MMP-2 -735C allele was associated with a 1.5-fold increase in IS incidence (OR=1.516, 95% CI=1.185-1.940, P=0.001), and MMP-9 -1562T allele was associated with a 1.5-fold increase in IS incidence (OR=1.543, 95% CI=1.144-2.080, P=0.004).
CONCLUSION
MMP-2 -735C and MMP-9 -1562T may act as IS susceptibility alleles.
目的
缺血性中风（IS）是由于脑部血流中断所致，可由血管中动脉粥样硬化斑块的形成引起。先前的研究表明基质金属蛋白酶（MMP）在斑块形成和中风风险中起作用。在此，我们探讨了两种MMP，即MMP-2和MMP-9与缺血性中风之间的关联。
方法
本研究纳入了396例缺血性中风患者和400名对照个体。通过对患者血样进行限制性片段长度多态性（RFLP）分析MMP-2 -1306C/T和-735C/T多态性以及MMP-9 -1562C/T多态性，并通过逻辑回归分析它们与缺血性中风的关系。
结果
MMP-2 -1306C/T的基因型和等位基因频率在统计学上无差异（P>0.05），但MMP-2 -735C/T和MMP-9 -1562C/T的基因型和等位基因频率在缺血性中风患者和对照样本之间存在差异（P<0.05）。虽然MMP-2 -1306C/T多态性与缺血性中风风险增加无关，但MMP-2 -735C等位基因与缺血性中风发病率增加1.5倍相关（OR=1.516，95%可信区间=1.185-1.940，P=0.001），MMP-9 -1562T等位基因与缺血性中风发病率增加1.5倍相关（OR=1.543，95%可信区间=1.144-2.080，P=0.004）。
结论
MMP-2 -735C和MMP-9 -1562T可能是缺血性中风的易感等位基因。
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