小鼠巨细胞病毒感染期间的抗病毒抗体依赖性细胞介导的细胞毒性
Antivirus antibody-dependent cell-mediated cytotoxicity during murine cytomegalovirus infection.
作者信息
Manischewitz J E, Quinnan G V
出版信息
Infect Immun. 1980 Sep;29(3):1050-4. doi: 10.1128/iai.29.3.1050-1054.1980.
BALB/c mice infected with murine cytomegalovirus were studied to determine whether antibody-dependent cell-mediated cytotoxicity contributes to the immune control of this infection. Antibody-dependent killer cells from uninfected mice were used as effector cells to assay for antibody in sera of infected mice. Secondary immune sera were found to contain both cytomegalovirus-specific and autoreactive antibodies. After primary infection only cytomegalovirus-specific antibodies were found. These were detected by antibody-dependent cell-mediated cytotoxicity within 8 to 10 days after onset of infection, but usually not until day 21, by a neutralizing antibody assay. Antibody titers were about 10-fold higher by antibody-dependent cell-mediated cytotoxicity than by neutralization. The results indicate that cellular immunity to cytomegalovirus infection includes an antibody-dependent cell-mediated cytotoxicity response which is likely to be highly efficient and may contribute significantly to control of both acute and later stages of infection.
对感染鼠巨细胞病毒的BALB/c小鼠进行了研究，以确定抗体依赖性细胞介导的细胞毒性是否有助于对这种感染的免疫控制。来自未感染小鼠的抗体依赖性杀伤细胞用作效应细胞，以检测感染小鼠血清中的抗体。发现二次免疫血清中同时含有巨细胞病毒特异性抗体和自身反应性抗体。初次感染后仅发现巨细胞病毒特异性抗体。这些抗体在感染开始后8至10天通过抗体依赖性细胞介导的细胞毒性检测到，但通常直到第21天通过中和抗体检测才能检测到。通过抗体依赖性细胞介导的细胞毒性检测到的抗体滴度比通过中和检测到的抗体滴度高约10倍。结果表明，对巨细胞病毒感染的细胞免疫包括抗体依赖性细胞介导的细胞毒性反应；这种反应可能非常有效，并可能对控制感染的急性期和后期阶段有显著贡献。
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