Shellam G R, Allan J E, Papadimitriou J M, Bancroft G J
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5104-8. doi: 10.1073/pnas.78.8.5104.
Mice homozygous for the beige gene (bg/bg) are a homologue of the Chédiak-Higashi syndrome of man and are known to be selectively defective in natural killer (NK) cells. We have compared the susceptibility of bg/bg and bg/+ C57BL/6J mice to infection with murine cytomegalovirus (MCMV). Beige mice are more susceptible to lethal infection and develop 33- to 43-fold higher virus titers in the liver, spleen, and kidney than do bg/+ mice after a sublethal infection, although virus replication is the same in vitro in cultured fibroblasts or epithelial cells from these mice. Inoculation with a sublethal dose of virus stimulates a NK cell response, although this is lower in bg/bg mice despite higher titers of interferon type 1 than in bg/+. A dose of MCMV that is lethal only to bg/bg augments cytotoxicity within 12 hr in bg/+ mice, whereas cytotoxicity in bg/bg remains very low. In bone marrow chimeras, recipients of bg/bg marrow were more susceptible to MCMV and had lower NK cell responses after virus inoculation than did recipients of marrow from bg/+ donors. The greater susceptibility of beige mice to the virus suggests that NK cells may contribute to resistance early in McMV infection.
米色基因纯合子（bg/bg）小鼠是人类切迪阿克-东综合征的同源物，已知其天然杀伤（NK）细胞存在选择性缺陷。我们比较了bg/bg和bg/+ C57BL/6J小鼠对鼠巨细胞病毒（MCMV）感染的易感性。米色小鼠对致死性感染更易感，在亚致死性感染后，其肝脏、脾脏和肾脏中的病毒滴度比bg/+小鼠高33至43倍，尽管这些小鼠的培养成纤维细胞或上皮细胞在体外的病毒复制情况相同。用亚致死剂量的病毒接种可刺激NK细胞反应，尽管bg/bg小鼠中的这种反应较低，尽管其1型干扰素滴度高于bg/+小鼠。仅对bg/bg致死的MCMV剂量在12小时内可增强bg/+小鼠的细胞毒性，而bg/bg小鼠中的细胞毒性仍然很低。在骨髓嵌合体中，接受bg/bg骨髓的受体比接受bg/+供体骨髓的受体对MCMV更易感，且病毒接种后NK细胞反应更低。米色小鼠对该病毒的易感性更高，这表明NK细胞可能在MCMV感染早期有助于抵抗感染。
