School of Physical and Mathematical Sciences, Nanyang Technological University , Singapore 637371.
Biochemistry. 2014 Mar 18;53(10):1595-600. doi: 10.1021/bi4015727. Epub 2014 Mar 6.
We investigate the structure formed by the first 18-nt of the 5'-terminal region of the human telomerase RNA (hTERC or hTR) using gel electrophoresis and UV, CD, and NMR spectroscopy. Our data suggest that this 18-nt sequence, r(GGGUUGCGGAGGGUGGGC), can form a stacked dimeric G-quadruplex in potassium solution. The two subunits, each being a three-layer parallel-stranded G-quadruplex with a cytosine bulge, are stacked at their 5'-end. The formation of this stacked dimeric G-quadruplex containing bulges could be biologically relevant for the dimerization and other interactions of the active human telomerase.
我们使用凝胶电泳和紫外、圆二色性、核磁共振波谱研究了人端粒酶 RNA（hTERC 或 hTR）5'-端区域的前 18 个核苷酸形成的结构。我们的数据表明，这个 18 个核苷酸序列 r(GGGUUGCGGAGGGUGGGC)可以在钾溶液中形成堆叠的二聚体 G-四链体。两个亚基，每个亚基都是一个具有胞嘧啶凸起的三层平行链 G-四链体，在其 5'-端堆叠。这种包含凸起的堆叠二聚体 G-四链体的形成可能与活性人端粒酶的二聚化和其他相互作用有关。
