Department of Chemical and Biological Engineering, Korea University , Seoul 136-701, Korea.
Biomacromolecules. 2014 Apr 14;15(4):1153-9. doi: 10.1021/bm401595q. Epub 2014 Mar 19.
Highly effective antifouling was achieved by immobilizing and stabilizing an acylase, disrupting bacterial cell-to-cell communication, in the form of cross-linked enzymes in magnetically separable mesoporous silica. This so-called "quorum-quenching" acylase (AC) was adsorbed into spherical mesoporous silica (S-MPS) with magnetic nanoparticles (Mag-S-MPS), and further cross-linked for the preparation of nanoscale enzyme reactors of AC in Mag-S-MPS (NER-AC/Mag-S-MPS). NER-AC effectively stabilized the AC activity under rigorous shaking at 200 rpm for 1 month, while free and adsorbed AC lost more than 90% of their initial activities in the same condition within 1 and 10 days, respectively. When applied to the membrane filtration for advanced water treatment, NER-AC efficiently alleviated the biofilm maturation of Pseudomonas aeruginosa PAO1 on the membrane surface, thereby enhancing the filtration performance by preventing membrane fouling. Highly stable and magnetically separable NER-AC, as an effective and sustainable antifouling material, has a great potential to be used in the membrane filtration for water reclamation.
通过将一种固定化和稳定化的酶（通过交联酶的形式固定在磁性可分离的介孔硅中，从而破坏细菌细胞间的通讯）用作抗污剂，实现了高效的抗污效果。这种所谓的“群体感应淬灭”酶（AC）被吸附到具有磁性纳米颗粒的球形介孔硅（Mag-S-MPS）中，并进一步交联，以制备 AC 在 Mag-S-MPS 中的纳米级酶反应器（NER-AC/Mag-S-MPS）。在 200rpm 剧烈搅拌下，NER-AC 可有效稳定 AC 的活性长达 1 个月，而在相同条件下，游离态和吸附态的 AC 在 1 天和 10 天内分别失去了超过 90%的初始活性。当应用于膜过滤进行高级水处理时，NER-AC 可有效缓解铜绿假单胞菌 PAO1 在膜表面的生物膜成熟，从而通过防止膜污染来提高过滤性能。高稳定性和可磁性分离的 NER-AC 作为一种有效且可持续的抗污材料，在用于水回收的膜过滤方面具有很大的应用潜力。
