Biol Chem. 2014 Jun;395(6):611-29. doi: 10.1515/hsz-2014-0116.
Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. The loading of Argonaute proteins with small RNAs is aided by a number of auxiliary factors as well as ATP hydrolysis. This review will focus on the mechanisms of Argonaute loading in different organisms. Furthermore, we highlight the versatile functions of small RNA-Argonaute protein complexes in organisms from all three kingdoms of life.
小 RNA 如 microRNAs（miRNAs）、短干扰 RNA（siRNAs）或 Piwi 相互作用 RNA（piRNAs）是各种生物中基因表达的重要调节剂。小 RNA 与 Argonaute 蛋白家族的成员结合，并被整合到更大的结构中，介导多种基因沉默事件。Argonaute 蛋白与小 RNA 的加载由许多辅助因子以及 ATP 水解辅助。这篇综述将重点介绍不同生物中 Argonaute 加载的机制。此外，我们还强调了小 RNA-Argonaute 蛋白复合物在所有三个生命王国的生物体中的多功能功能。
