Cyr61 的表达与结直肠癌患者的预后相关。
Cyr61 expression is associated with prognosis in patients with colorectal cancer.
机构信息
Department of Surgery, College of Medicine, Soonchunhyang University, 31 soonchunhyang 6 gil, Dongnam-gu, Cheonan, Chungcheongnam-do 330-722, Republic of Korea.
出版信息
BMC Cancer. 2014 Mar 7;14:164. doi: 10.1186/1471-2407-14-164.
BACKGROUND
Cysteine-rich 61 (Cyr61), a member of the CCN protein family, possesses diverse functionality in cellular processes such as adhesion, migration, proliferation, and survival. Cyr61 can also function as an oncogene or a tumour suppressor, depending on the origin of the cancer. Only a few studies have reported Cyr61 expression in colorectal cancer. In this study, we assessed the Cyr61 expression in 251 colorectal cancers with clinical follow up.
METHODS
We examined Cyr61 expression in 6 colorectal cancer cell lines (HT29, Colo205, Lovo, HCT116, SW480, SW620) and 20 sets of paired normal and colorectal cancer tissues by western blot. To validate the association of Cyr61 expression with clinicopathological parameters, we assessed Cyr61 expression using tissue microarray analysis of primary colorectal cancer by immunohistochemical analysis.
RESULTS
We verified that all of the cancer cell lines expressed Cyr61; 2 cell lines (HT29 and Colo205) demonstrated Cyr61 expression to a slight extent, while 4 cell lines (Lovo, HCT116, SW480, SW620) demonstrated greater Cyr61 expression than HT29 and Colo205 cell lines. Among the 20 cases of paired normal and tumour tissues, greater Cyr61 expression was observed in 16 (80%) tumour tissues than in normal tissues. Furthermore, 157 out of 251 cases (62.5%) of colorectal cancer examined in this study displayed strong Cyr61 expression. Cyr61 expression was found to be associated with pN (p = 0.018). Moreover, Cyr61 expression was associated with statistically significant cancer-specific mortality (p = 0.029). The duration of survival was significantly lesser in patients with Cyr61 high expression than in patients with Cyr61 low expression (p = 0.001). These results suggest that Cyr61 expression plays several important roles in carcinogenesis and may also be a good prognostic marker for colorectal cancer.
CONCLUSIONS
Our data confirmed that Cyr61 was expressed in colorectal cancers and the expression was correlated with worse prognosis of colorectal cancers.
背景
富含半胱氨酸的 61 型（Cyr61）是细胞外基质细胞因子 CCN 蛋白家族的一员，在细胞黏附、迁移、增殖和存活等多种细胞过程中具有多种功能。Cyr61 可以作为癌基因或肿瘤抑制因子发挥作用，具体取决于癌症的起源。仅有少数研究报告了结直肠癌中 Cyr61 的表达。在本研究中，我们评估了 251 例具有临床随访的结直肠癌中 Cyr61 的表达情况。
方法
我们通过 Western blot 检测了 6 种结直肠癌细胞系（HT29、Colo205、Lovo、HCT116、SW480、SW620）和 20 对配对的正常和结直肠癌细胞组织中 Cyr61 的表达。为了验证 Cyr61 表达与临床病理参数的相关性，我们通过免疫组织化学分析对原发性结直肠癌的组织微阵列进行了 Cyr61 表达分析。
结果
我们验证了所有的癌细胞系都表达了 Cyr61；2 个细胞系（HT29 和 Colo205）表达程度较轻，而 4 个细胞系（Lovo、HCT116、SW480、SW620）的表达程度高于 HT29 和 Colo205 细胞系。在 20 对正常和肿瘤组织中，16 例（80%）肿瘤组织中的 Cyr61 表达高于正常组织。此外，在本研究中检查的 251 例结直肠癌病例中，有 157 例（62.5%）表现出强烈的 Cyr61 表达。Cyr61 表达与 pN 相关（p=0.018）。此外，Cyr61 表达与结直肠癌特异性死亡率显著相关（p=0.029）。Cyr61 高表达患者的生存时间明显短于 Cyr61 低表达患者（p=0.001）。这些结果表明，Cyr61 的表达在结直肠癌的发生中发挥了重要作用，并且可能也是结直肠癌的一个良好预后标志物。
结论
我们的数据证实 Cyr61 在结直肠癌中表达，并与结直肠癌的预后较差相关。
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