Zhao Kongyin, Lin Beibei, Cui Wenkui, Feng Lingzhi, Chen Tian, Wei Junfu
State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China; School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China; School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
Talanta. 2014 Apr;121:256-62. doi: 10.1016/j.talanta.2014.01.010. Epub 2014 Jan 18.
Bovine serum albumin (BSA) imprinted polypropylene (PP) fiber-grafted polyacrylamide (PAM) hydrogel membrane (PP-g-PAM MIP) was prepared using non-woven PP fiber as matrix, BSA as template molecule, and acrylamide (AM) as functional monomer via UV radiation-reduced polymerization in an aqueous phase. SEM, FT-IR, DSC and TG were used to characterize the PP grafted PAM hydrogel. Influence factors on the adsorption capacity of PP-g-PAM MIP were investigated, such as monomer concentration, cross-linker concentration, template molecule amount and pH values in BSA solution. The adsorption and recognition properties of PP-g-PAM MIP were evaluated and the results showed that the PP-g-PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA as compared with non-imprinted ones. PP-g-PAM MIPs could recognize the template protein using Lys, Ova, BHb, and Glo as control proteins, and the selectivity factor (β) was above 2.0. The imprinting efficiency of PP-g-PAM MIP tended to be stable after three cycles and maintained 76% of the initial value of the imprinting efficiency even after five repetitions, which was more excellent than that of PAM microsphere. The PP-g-PAM MIP is low cost and easy to be prepared, which would show its potential applications in the fields of extracting and testing required proteins from cells or particulate samples.
以聚丙烯（PP）无纺布纤维为基质、牛血清白蛋白（BSA）为模板分子、丙烯酰胺（AM）为功能单体，通过水相紫外辐射还原聚合反应制备了牛血清白蛋白印迹聚丙烯（PP）纤维接枝聚丙烯酰胺（PAM）水凝胶膜（PP-g-PAM MIP）。采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、差示扫描量热法（DSC）和热重分析法（TG）对PP接枝PAM水凝胶进行了表征。研究了单体浓度、交联剂浓度、模板分子用量和BSA溶液pH值等因素对PP-g-PAM MIP吸附性能的影响。对PP-g-PAM MIP的吸附和识别性能进行了评价，结果表明，与非印迹膜相比，PP-g-PAM MIP对BSA的吸附性能有明显提高。以赖氨酸（Lys）、卵清蛋白（Ova）、血红蛋白（BHb）和球蛋白（Glo）为对照蛋白，PP-g-PAM MIPs能够识别模板蛋白，选择性因子（β）大于2.0。PP-g-PAM MIP的印迹效率在3次循环后趋于稳定，即使经过5次重复，仍保持初始印迹效率的76%，优于PAM微球。PP-g-PAM MIP成本低、易于制备，在从细胞或颗粒样品中提取和检测所需蛋白质领域具有潜在应用前景。
