School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, China.
J Chromatogr A. 2011 Jun 3;1218(22):3489-95. doi: 10.1016/j.chroma.2011.03.069. Epub 2011 Apr 4.
Currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. In this work, bovine serum albumin (BSA) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (ATRP) method in the presence of common monomer (N-isopropylacrylamide) with the assistant of basic functional monomer (N-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the ATPR with the mild reaction conditions. The BSA-imprinted polymer exhibited higher adsorption capacity and selectivity to BSA over the non-imprinted polymer. Competitive adsorption tests indicated the BSA-imprinted polymer had better selective adsorption and recognition properties to BSA in the mixture. The obtained BSA-imprinted polymer was applied to bovine serum, which also showed selectivity to BSA. In addition, a conventional aqueous two-phase solution of PEG/sulphate was used as elution for adsorbed BSA, which was compared with common NaCl elution.
目前，小分子印迹的报道较多，因为大分子印迹由于其体积大和复杂结构而面临挑战。在这项工作中，基于原子转移自由基聚合（ATRP）方法，在常见单体（N-异丙基丙烯酰胺）的存在下，辅助碱性功能单体（N-[3-（二甲基氨基）丙基] - 甲基丙烯酰胺）成功合成了牛血清白蛋白（BSA）表面印迹磁性聚合物，为基于温和反应条件的 ATRP 印迹更大的目标蛋白提供了可行的尝试。与非印迹聚合物相比，BSA 印迹聚合物对 BSA 表现出更高的吸附容量和选择性。竞争吸附试验表明，BSA 印迹聚合物在混合物中对 BSA 具有更好的选择性吸附和识别性能。所得的 BSA 印迹聚合物被应用于牛血清中，也表现出对 BSA 的选择性。此外，还使用常规的 PEG/硫酸盐双水相溶液作为吸附 BSA 的洗脱液，与常用的 NaCl 洗脱液进行了比较。
