一种通过碱性洗脱和微量荧光法测定DNA来检测哺乳动物细胞中DNA损伤的方法。
A procedure for the assay of DNA damage in mammalian cells by alkaline elution and microfluorometric DNA determination.
作者信息
Brambilla G, Cavanna M, Sciabà L, Carlo P, Parodi S, Taningher M
出版信息
Ital J Biochem. 1977 Nov-Dec;26(6):419-27.
PMID:24609
Abstract
The microfluorimetric assay of Kissane and Robins (J. Biol. Chem. 233, 184-188, 1958) has been modified to monitor DNA content in alkaline elution fractions. The elution profiles of DNA from treated and control cultured cells demonstrated a good correlation whether DNA in the elution fractions was determined by the fluorometric method or by measuring its radioactivity. The DNA recovery was of about 80% and the measured material was DNase digestible.
摘要
基桑和罗宾斯（《生物化学杂志》233卷，第184 - 188页，1958年）的微量荧光测定法已被改进，用于监测碱性洗脱组分中的DNA含量。无论是通过荧光法还是通过测量其放射性来测定洗脱组分中的DNA，处理过的培养细胞和对照培养细胞的DNA洗脱曲线都显示出良好的相关性。DNA回收率约为80%，且所测物质可被DNA酶消化。
Zotero 插件