Abbas Naeem, Khan Hafiz Azhar Ali, Shad Sarfraz Ali
Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan,
Ecotoxicology. 2014 Jul;23(5):791-801. doi: 10.1007/s10646-014-1217-7. Epub 2014 Mar 8.
Lambda-cyhalothrin, a pyrethroid insecticide, has been used frequently for the control of house flies, Musca domestica L., worldwide including Pakistan. To assess the resistance risk and design a resistance management strategy, a house fly population was exposed to lambda-cyhalothrin in the laboratory to assess inheritance and heritability, and cross-resistance to other insecticides, including different chemical classes. After 11 generations of selection, the population developed 113.57-fold resistance to lambda-cyhalothrin compared to the susceptible population. There was no cross-resistance to bifenthrin and methomyl, but very low cross-resistance to abamectin and indoxacarb in the lambda-cyhalothrin selected population compared to the field population. Synergism bioassay with piperonyl butoxide and S,S,S-tributylphosphorotrithioate indicated that lambda-cyhalothrin resistance was associated with microsomal oxidases and esterases. The LC50 values of F1 (Lambda-SEL ♀ × Susceptible ♂) and F'1 (Lambda-SEL ♂ × Susceptible ♀) populations were not significantly different and dominance (DLC) values were 0.68 and 0.62. The resistance to lambda-cyhalothrin was completely recessive (DML = 0.00) at highest dose and completely dominant at lowest dose (DML = 0.95). The monogenic model of inheritance showed that lambda-cyhalothrin resistance was controlled by multiple factors. The heritability values were 0.20, 0.04, 0.003, 0.07 and 0.08 for lambda-cyhalothrin, bifenthrin, methomyl, indoxacarb and abamectin resistance, respectively. It was concluded that lambda-cyhalothrin resistance in house flies was autosomally inherited, incompletely dominant and controlled by multiple factors. These findings would be helpful to improve the management of house flies.
高效氯氟氰菊酯是一种拟除虫菊酯类杀虫剂，在包括巴基斯坦在内的全球范围内，已被频繁用于控制家蝇（Musca domestica L.）。为评估抗性风险并制定抗性管理策略，在实验室中让一群家蝇接触高效氯氟氰菊酯，以评估其遗传和遗传力，以及对包括不同化学类别的其他杀虫剂的交叉抗性。经过11代选育后，该群体对高效氯氟氰菊酯的抗性相较于敏感群体提高了113.57倍。与联苯菊酯和灭多威不存在交叉抗性，但相较于田间群体，在高效氯氟氰菊酯选育群体中，与阿维菌素和茚虫威存在非常低的交叉抗性。用胡椒基丁醚和三丁基三硫代磷酸酯进行增效生物测定表明，高效氯氟氰菊酯抗性与微粒体氧化酶和酯酶有关。F1（高效氯氟氰菊酯选育♀×敏感♂）和F'1（高效氯氟氰菊酯选育♂×敏感♀）群体的LC50值无显著差异，显性度（DLC）值分别为0.68和0.62。在最高剂量下，对高效氯氟氰菊酯的抗性完全隐性（DML = 0.00），在最低剂量下完全显性（DML = 0.95）。单基因遗传模型表明，高效氯氟氰菊酯抗性受多个因素控制。高效氯氟氰菊酯、联苯菊酯、灭多威、茚虫威和阿维菌素抗性的遗传力值分别为0.20、0.04、0.003、0.07和0.08。得出的结论是，家蝇对高效氯氟氰菊酯的抗性是常染色体遗传，不完全显性且受多个因素控制。这些发现将有助于改进家蝇的管理。
