Singh V K, Maheshwari R K, Damewood G P, Stephensen C B, Oliver C, Friedman R M
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD.
J Biol Regul Homeost Agents. 1988 Apr-Jun;2(2):53-62.
Double-label immunofluorescence staining studies in virus-infected subclone 11 of LB cells indicated that almost all of the vesicular stomatitis virus (VSV) glycoprotein (G) was plasma membrane-associated during the logarithmic phase of virus replication. In contrast, treatment with interferon (IFN) resulted in inhibition of VSV-G transport, so that almost all of the G remained associated with the Golgi complex (GC) at comparable times after infection. In both IFN-treated and control cells, G was resistant to treatment with the enzyme endo-beta-N-acetylglucosamine H (endo H) indicating that the bulk of the G had reached the trans compartment of the GC.
在病毒感染的LB细胞亚克隆11中进行的双标记免疫荧光染色研究表明，在病毒复制的对数期，几乎所有水泡性口炎病毒（VSV）糖蛋白（G）都与质膜相关。相比之下，用干扰素（IFN）处理导致VSV-G转运受到抑制，因此在感染后相当的时间，几乎所有的G仍与高尔基体复合体（GC）相关。在IFN处理的细胞和对照细胞中，G对β-N-乙酰葡糖胺内切酶H（内切酶H）处理具有抗性，这表明大部分G已到达GC的反式区室。
