Alizadeh AliReza, Esmaeili Vahid, Shahverdi Abdolhossein, Rashidi Ladan
Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh, Iran.
Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Cell J. 2014 Fall;16(3):289-98. Epub 2014 Oct 4.
The effects of dietary fish oil on semen quality and sperm fatty acid profiles during consumption of n-3 fatty acids as well as the persistency of fatty acids in ram's sperm after removing dietary oil from the diet were investigated.
In this experimental study, we randomly assigned 9 Zandi rams to two groups (isoenergetic and isonitrogenous diets): control (CTR; n=5) and fish oil (FO; n=4) for 70 days with a constant level of vitamin E in both groups. Semen was collected at the first week and at the last week of the feeding period (phase 1). After the feeding period, all rams were fed a conventional diet and semen samples were collected one and two months after removal of FO (phase 2). The sperm parameters and fatty acid profiles were measured by computer assisted semen analyzer (CASA) and gas chromatography (GC), respectively. The completely randomized design was used and data were analyzed with SPSS version 16.
Dietary FO had significant positive effects on all sperm quality and quantity parameters compared with the CTR during the feeding period (p<0.05). The positive effects of FO on sperm concentration and total sperm output were observed at one and two months after removal of FO (p<0.05), whereas other sperm parameters were unaffected. Before feeding, C14 (myristic acid), C16 (palmitic acid), C18 (stearic acid), C18:1 (oleic acid) and C22:6 (docosahexaenoic acid: DHA) were the primary sperm FA. FO in the diet increased sperm DHA, C14:0 and C18:0 during the feeding period (p<0.05).
The present study showed not only manipulation of ram sperm fatty acid profiles by dietary FO and sperm parameters during the feeding period, but also the persistency of unique effects of dietary omega-3 fatty acids up to two months following its removal from the diet. Also, we recommend that sperm fatty acid profiles should be comprehensively analyzed and monitored.
研究在摄入n-3脂肪酸期间，日粮鱼油对精液质量和精子脂肪酸谱的影响，以及从日粮中去除鱼油后，公羊精子中脂肪酸的持续性。
在本实验研究中，我们将9只赞迪公羊随机分为两组（等能量和等氮日粮）：对照组（CTR；n = 5）和鱼油组（FO；n = 4），为期70天，两组维生素E水平恒定。在饲喂期的第一周和最后一周（阶段1）采集精液。饲喂期结束后，所有公羊改为常规日粮，并在去除鱼油后1个月和2个月采集精液样本（阶段2）。分别通过计算机辅助精液分析仪（CASA）和气相色谱法（GC）测量精子参数和脂肪酸谱。采用完全随机设计，数据用SPSS 16版软件进行分析。
在饲喂期，与对照组相比，日粮鱼油对所有精子质量和数量参数均有显著的积极影响（p<0.05）。在去除鱼油后1个月和2个月时，观察到鱼油对精子浓度和总精子产量有积极影响（p<0.05），而其他精子参数未受影响。饲喂前，C14（肉豆蔻酸）、C16（棕榈酸）、C18（硬脂酸）、C18:1（油酸）和C22:6（二十二碳六烯酸：DHA）是精子中的主要脂肪酸。日粮中的鱼油在饲喂期增加了精子中的DHA、C14:0和C18:0（p<0.05）。
本研究表明，日粮鱼油不仅在饲喂期可调控公羊精子脂肪酸谱和精子参数，而且在从日粮中去除鱼油后长达两个月，日粮ω-3脂肪酸的独特作用仍具持续性。此外，我们建议应全面分析和监测精子脂肪酸谱。
