Roksandić-Krizan Ivana, Bosnjak Zinka, Perić Magdalena, Durkin Ivona, Atalić Vlasta Zujić, Vuković Dubravka
Institute for Public Health for Osijek-Baranja County, Department of Microbiology, Osijek, Croatia.
University Josip Juraj Strossmayer, Faculty of Agriculture, Osijek, Croatia.
Coll Antropol. 2013 Dec;37(4):1179-83.
Genital infection with high-risk human papillomavirus (HR HPV) associates with increased risk of developing precancerous lesions, such as cervical intraepithelial neoplasia (CIN). The objective of this pilot study conducted in north-east Croatia was to determine the prevalence of HPV genital infection in women with abnormal cervical cytology and to determine its association with their age and HPV genotype(s). From March 2009 to December 2011, cervical swabs from 100 women were analysed for HR HPV infection (AMPLICOR HPV Test, Roche Diagnostics) and genotyped for high risk (HR), intermediate (IR) and low risk (LR) HPVs (Linear Array HPV Genotyping Test, Roche Diagnostics). The most prevalent HR genotypes in women with CIN were HPV 16 (27.6%), HPV 31 (11.8%), HPV 51 and HPV 52 (10.2% each). The most prevalent IR genotypes were HPV 66 (30%) and HPV 62 (23.3%). The most prevalent LR genotype was HPV 6 (20.3%). Women between 21 and 25 years of age showed the highest rate of HPV infection (44.2%). Moreover, women younger than 35 years showed a significant association (p < 0.01) and positive correlation (r = 0.67; p < 0.05) between HR HPV infection and CIN stages 1 and 2. Multiple HPV infections were found in almost half of the women. This is the first study that analysed the prevalence of genital infection with HR/IR/LR HPVs in women with CIN from north-east Croatia. Despite the preliminary nature of this pilot study, the lower prevalence of some HR HPVs (HPV18) and the higher prevalence of other HR HPVs (HPVs 51, 52 and 31) may imply the necessity for the development of more targeted anti-HPV vaccines or other strategies for more efficient protection against oncogenic HPV infection in women from our region.
高危型人乳头瘤病毒（HR HPV）生殖器感染会增加患癌前病变的风险，如宫颈上皮内瘤变（CIN）。在克罗地亚东北部开展的这项初步研究的目的是确定宫颈细胞学异常女性中HPV生殖器感染的患病率，并确定其与年龄及HPV基因型的关联。2009年3月至2011年12月，对100名女性的宫颈拭子进行HR HPV感染分析（COBAS TaqMan HPV检测，罗氏诊断），并对高危（HR）、中危（IR）和低危（LR）HPV进行基因分型（线性阵列HPV基因分型检测，罗氏诊断）。CIN女性中最常见的HR基因型是HPV 16（27.6%）、HPV 31（11.8%）、HPV 51和HPV 52（各10.2%）。最常见的IR基因型是HPV 66（30%）和HPV 62（23.3%）。最常见的LR基因型是HPV 6（20.3%）。21至25岁的女性HPV感染率最高（44.2%）。此外，35岁以下女性中，HR HPV感染与CIN 1期和2期之间存在显著关联（p < 0.01）和正相关（r = 0.67；p < 0.05）。几乎一半的女性存在多种HPV感染。这是第一项分析克罗地亚东北部CIN女性中HR/IR/LR HPV生殖器感染患病率的研究。尽管这项初步研究具有初步性质，但某些HR HPV（HPV18）的较低患病率和其他HR HPV（HPV 51、52和31）的较高患病率可能意味着有必要研发更具针对性的抗HPV疫苗或其他策略，以便更有效地保护我们地区的女性免受致癌性HPV感染。
