Crow J, More L, Howe S
Department of Histopathology, Royal Free Hospital, London, England.
APMIS. 1988 Oct;96(10):921-6. doi: 10.1111/j.1699-0463.1988.tb00963.x.
Staining of mast cells in the human uterus has been studied using four fixatives and five staining methods to determine whether there are subpopulations of mucosal (endometrial) and connective tissue (myometrial) mast cells, and to discover how they can best be demonstrated. Following formalin fixation none of the staining methods showed maximum staining of mast cells in either endometrium or myometrium. The best demonstration of uterine mast cells is by fixation with either isotonic formol acetic acid or Mota's basic lead acetate followed by staining with the long toluidine blue technique. Although the degree of MC understaining following formalin fixation was greater for the endometrium than for the myometrium this is inadequate evidence to designate two cell populations. The findings suggest that the mast cells of the human uterus are all one population but show heterogeneity of histological properties possibly related to their functional state.
利用四种固定剂和五种染色方法对人子宫中的肥大细胞进行了染色研究，以确定黏膜（子宫内膜）和结缔组织（子宫肌层）肥大细胞是否存在亚群，并探索如何能最佳地显示它们。经福尔马林固定后，所有染色方法均未在子宫内膜或子宫肌层中显示肥大细胞的最大染色效果。子宫肥大细胞的最佳显示方法是先用等渗甲醛乙酸或莫塔氏碱性醋酸铅固定，然后用长效甲苯胺蓝技术染色。尽管福尔马林固定后子宫内膜中肥大细胞染色不足的程度比子宫肌层更大，但这不足以证明存在两种细胞群。研究结果表明，人子宫中的肥大细胞均为单一群体，但显示出可能与其功能状态相关的组织学特性异质性。
