Liu Mingjiang, Song Shixiu, Li Huanrong, Jiang Xiaoyu, Yin Peng, Wan Changrong, Liu Xiaoxi, Liu Fenghua, Xu Jianqin
College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, P. R. China.
College of Animal Science and Technology, Beijing University of Agriculture (BUA), Beijing 102206, P. R. China.
J Dairy Sci. 2014 May;97(5):2856-65. doi: 10.3168/jds.2013-7600. Epub 2014 Mar 5.
Caffeic acid possesses multiple biological effects, such as antibacterial, antioxidant, antiinflammatory, and anticancer growth; however, what effects it has on bovine mastitis have not been investigated. The aim of this study was to verify the antiinflammatory properties of caffeic acid on the inflammatory response of primary bovine mammary epithelial cells (bMEC) induced by lipopolysaccharide (LPS), and to clarify the possible underlying mechanism. Bovine mammary epithelial cells were treated with various concentrations (10, 50, 100, and 200 μg/mL) of LPS for 3, 6, 12, and 18 h; the results showed that LPS significantly inhibited cell viability in a time- and dose-dependent manner. When cells were treated with LPS (50 μg/mL) for 12h, the cell membrane permeability significantly increased, which promoted cell apoptosis. Various concentrations (10, 25, and 50 μg/mL) of caffeic acid could weaken the inflammation injury of bMEC induced by LPS without cytotoxicity. Proinflammatory cytokines (IL-8, IL-1β, IL-6, and tumor necrosis factor α) from bMEC were decreased. Nuclear transcription factor κB activity was weakened via blocking κB inhibitor α degradation and p65 phosphorylation. All these showed that the protective effect of caffeic acid on LPS-induced inflammation injury in bMEC was at least partly achieved by the decreased production of proinflammatory cytokines mediated by the effect of reducing the κB inhibitor α degradation and p65 phosphorylation in the nuclear transcription factor κB pathway. The use of caffeic acid would be beneficial in dairy cows during Escherichia coli mastitis as a safe and natural antiinflammatory drug.
咖啡酸具有多种生物学效应，如抗菌、抗氧化、抗炎和抗癌生长等；然而，其对牛乳腺炎有何影响尚未得到研究。本研究的目的是验证咖啡酸对脂多糖（LPS）诱导的原代牛乳腺上皮细胞（bMEC）炎症反应的抗炎特性，并阐明可能的潜在机制。用不同浓度（10、50、100和200μg/mL）的LPS处理牛乳腺上皮细胞3、6、12和18小时；结果表明，LPS以时间和剂量依赖性方式显著抑制细胞活力。当细胞用LPS（50μg/mL）处理12小时时，细胞膜通透性显著增加，从而促进细胞凋亡。不同浓度（10、25和50μg/mL）的咖啡酸可减轻LPS诱导的bMEC炎症损伤且无细胞毒性。bMEC产生的促炎细胞因子（IL-8、IL-1β、IL-6和肿瘤坏死因子α）减少。通过阻断κB抑制剂α降解和p65磷酸化，核转录因子κB活性减弱。所有这些表明，咖啡酸对LPS诱导的bMEC炎症损伤的保护作用至少部分是通过减少核转录因子κB途径中κB抑制剂α降解和p65磷酸化的作用介导的促炎细胞因子产生减少来实现的。在大肠杆菌性乳腺炎期间，将咖啡酸作为一种安全天然的抗炎药物用于奶牛将是有益的。
