Yin Fei, Guo Li, Meng Chun-yang, Liu Ya-juan, Lu Ri-feng, Li Peng, Zhou Yu-bo
Department of Spine Surgery, First Hospital of Jilin University, Changchun 130021, Jilin, PR China.
Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, Jilin, PR China.
Brain Res. 2014 May 2;1561:1-10. doi: 10.1016/j.brainres.2014.02.047. Epub 2014 Mar 5.
It is unknown whether transplantation of bone marrow mesenchymal stem cells (BM-MSCs) can repair spinal cord ischemia-reperfusion injury (SCII) in a rat model through an anti-apoptotic effect. Adult rats were divided into untreated or sham-operated controls, untreated models of SCII (uSCII) and BM-MSC-transplanted models of SCII (tSCII; labeled with CM-Dill transplanted at 1 h and 24 h after reperfusion). According to evaluation of hind-limb motor function, the motor functions of tSCII rats were significantly better than those of uSCII rats by the seventh day. H&E and TUNEL staining showed that the spinal cords of uSCII rats contained damaged neural cells with nuclear pyknosis and congestion of blood vessels, with a high percentage of apoptotic neural cells, while the spinal cords of tSCII rats were nearly normal with significantly fewer apoptotic neural cells. Immunohistochemistry and double immunofluorescence staining revealed that in tSCII rats CASP3 and neurofilament-H (NF-H) levels were 14.57% and 174% those of uSCII rats, respectively, and in tSCII rats the ratio of BAX to BCL2 was reduced by nearly 50%. The differentiation of transplanted CM-Dil-labeled BM-MSCs into neurons and astrocytes was observed in the spinal cords of the tSCII rats under laser scanning confocal microscopy. These results showed that transplantation of BM-MSCs improved functional recovery after SCII via anti-apoptosis.
骨髓间充质干细胞（BM-MSCs）移植是否能通过抗凋亡作用修复大鼠模型中的脊髓缺血再灌注损伤（SCII）尚不清楚。成年大鼠被分为未治疗或假手术对照组、未治疗的SCII模型（uSCII）和BM-MSC移植的SCII模型（tSCII；在再灌注后1小时和24小时用CM-Dil标记进行移植）。根据后肢运动功能评估，到第7天时，tSCII大鼠的运动功能明显优于uSCII大鼠。苏木精-伊红（H&E）和TUNEL染色显示，uSCII大鼠脊髓中存在神经细胞损伤，表现为核固缩和血管充血，凋亡神经细胞百分比高，而tSCII大鼠脊髓几乎正常，凋亡神经细胞明显减少。免疫组织化学和双重免疫荧光染色显示，在tSCII大鼠中，半胱天冬酶3（CASP3）和神经丝-H（NF-H）水平分别为uSCII大鼠的14.57%和174%，且在tSCII大鼠中，BAX与BCL2的比值降低了近50%。在激光扫描共聚焦显微镜下观察到，tSCII大鼠脊髓中移植的CM-Dil标记的BM-MSCs分化为神经元和星形胶质细胞。这些结果表明，BM-MSCs移植通过抗凋亡作用改善了SCII后的功能恢复。
