Pinet F, Corvol M T, Bourguignon J, Corvol P
INSERM U36, Paris, France.
J Clin Endocrinol Metab. 1988 Dec;67(6):1211-20. doi: 10.1210/jcem-67-6-1211.
Chorionic tissue is one of the major extrarenal sites of renin production, and as such, cultured chorionic cells are a potential model for in vitro studies of renin biosynthesis and regulation. Human chorionic cells were isolated from four chorions and maintained in tissue culture for a total of eight subcultures. Total renin production was considerable in the primary cultures, but fell gradually with successive passages. The cells could be frozen and thawed without losing their ability to divide or produce renin. Both the primary cultures and the subcultures contained a single type of elongated cell containing abundant rough endoplasmic reticulum and myofibrils, but no renin granules, suggesting that the cells had smooth muscle-like features. Immunocytochemistry indicated that they contained both renin and prorenin. The renin produced by the chorionic cells was not stored within the cells, but was released rapidly into the medium. More than 95% of the renin produced was prorenin, which, after activation, had biochemical and immunological properties similar to those of pure human renin. The cells contained a renin mRNA that had the same size as that for renal renin (1.6 kilobases), confirming the synthesis of renin by these cells. The cells were also examined for the presence of other components of the renin-angiotensin system. Angiotensinogen and angiotensin I were not detected, but angiotensin-converting enzyme was present in extracts of primary and secondary cultured cells. beta hCG and progesterone were also found in the medium of primary culture. However, the production of beta hCG and progesterone fell after the primary culture, and beta hCG and progesterone were indetectable in secondary and tertiary cultures, respectively. These experiments suggest that these two hormones do not influence renin synthesis or vice versa. Thus, these cultures of human chorionic cells synthesized considerable quantities of prorenin and can provide a permanent source of nonrenal prorenin-producing cells.
绒毛膜组织是肾素产生的主要肾外部位之一，因此，培养的绒毛膜细胞是肾素生物合成和调节体外研究的潜在模型。从四个绒毛膜中分离出人类绒毛膜细胞，并在组织培养中进行了总共八次传代培养。原代培养中肾素的总产量相当可观，但随着连续传代逐渐下降。细胞可以冷冻和解冻，而不会失去其分裂或产生肾素的能力。原代培养和传代培养均含有单一类型的细长细胞，这些细胞含有丰富的粗面内质网和肌原纤维，但没有肾素颗粒，这表明这些细胞具有平滑肌样特征。免疫细胞化学表明它们同时含有肾素和肾素原。绒毛膜细胞产生的肾素不储存在细胞内，而是迅速释放到培养基中。产生的肾素中超过95%是肾素原，激活后，其生化和免疫特性与纯人肾素相似。细胞含有与肾肾素大小相同（1.6千碱基）的肾素mRNA，证实了这些细胞合成了肾素。还检测了这些细胞中肾素-血管紧张素系统其他成分的存在情况。未检测到血管紧张素原和血管紧张素I，但原代和二代培养细胞提取物中存在血管紧张素转换酶。原代培养的培养基中也发现了β-hCG和孕酮。然而，原代培养后β-hCG和孕酮的产量下降，在二代和三代培养中分别检测不到β-hCG和孕酮。这些实验表明这两种激素不影响肾素合成，反之亦然。因此，这些人类绒毛膜细胞培养物合成了大量肾素原，并可提供产生非肾性肾素原细胞的永久来源。
