许多聚合酶中的一种序列基序。
A sequence motif in many polymerases.
作者信息
Argos P
机构信息
European Molecular Biology Laboratory, Heidelberg, FRG.
出版信息
Nucleic Acids Res. 1988 Nov 11;16(21):9909-16. doi: 10.1093/nar/16.21.9909.
Abstract
A 15-residue sequence motif has been found in many polymerases from various species and involving DNA and RNA dependence and product. The motif is characterized by a Tyr-Gly-Asp-(Thr)-Asp core flanked by hydrophobic spans five residues in length. An mRNA maturase segment is also suggested to display the motif pattern. The aspartates may be important in polymerase function by acting directly in catalysis and/or by binding magnesium.
摘要
在来自不同物种的许多聚合酶中发现了一个15个残基的序列基序，涉及DNA和RNA依赖性以及产物。该基序的特征是一个酪氨酸-甘氨酸-天冬氨酸-（苏氨酸）-天冬氨酸核心，两侧是长度为五个残基的疏水跨度。还表明一个mRNA成熟酶片段也呈现该基序模式。天冬氨酸可能通过直接参与催化和/或结合镁在聚合酶功能中起重要作用。
相似文献
1
A sequence motif in many polymerases.许多聚合酶中的一种序列基序。
Nucleic Acids Res. 1988 Nov 11;16(21):9909-16. doi: 10.1093/nar/16.21.9909.
2
Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses.植物、动物和细菌病毒的RNA依赖性聚合酶的一级结构比较。
Nucleic Acids Res. 1984 Sep 25;12(18):7269-82. doi: 10.1093/nar/12.18.7269.
3
The highly conserved amino acid sequence motif Tyr-Gly-Asp-Thr-Asp-Ser in alpha-like DNA polymerases is required by phage phi 29 DNA polymerase for protein-primed initiation and polymerization.α样DNA聚合酶中高度保守的氨基酸序列基序Tyr-Gly-Asp-Thr-Asp-Ser是噬菌体φ29 DNA聚合酶进行蛋白质引发起始和聚合所必需的。
Proc Natl Acad Sci U S A. 1990 Jun;87(12):4610-4. doi: 10.1073/pnas.87.12.4610.
4
Effects of substitutions in a conserved DX(2)GR sequence motif, found in many DNA-dependent nucleotide polymerases, on transcription by T7 RNA polymerase.在许多依赖DNA的核苷酸聚合酶中发现的保守DX(2)GR序列基序中的取代对T7 RNA聚合酶转录的影响。
J Mol Biol. 2002 May 24;319(1):37-51. doi: 10.1016/S0022-2836(02)00261-9.
5
An amino acid sequence motif linking viral DNA polymerases and plant virus proteins involved in RNA replication.一种连接病毒DNA聚合酶与参与RNA复制的植物病毒蛋白的氨基酸序列基序。
Nucleic Acids Res. 1986 Aug 26;14(16):6769. doi: 10.1093/nar/14.16.6769.
6
Two Neurospora mitochondrial plasmids encode DNA polymerases containing motifs characteristic of family B DNA polymerases but lack the sequence Asp-Thr-Asp.
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4299-303. doi: 10.1073/pnas.90.9.4299.
7
Bacteriophage PRD1 DNA polymerase: evolution of DNA polymerases.噬菌体PRD1 DNA聚合酶：DNA聚合酶的进化
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8287-91. doi: 10.1073/pnas.84.23.8287.
8
[Genetic system of selecting point mutations of bacteriophage T7 RNA polymerase].[噬菌体T7 RNA聚合酶点突变的遗传选择系统]
Mol Biol (Mosk). 1991 Nov-Dec;25(6):1588-93.
9
Genes coding for reverse transcriptase, DNA-directed RNA polymerase, and chitin synthase from the microsporidian Spraguea lophii.
Biol Bull. 1997 Oct;193(2):250-1. doi: 10.1086/BBLv193n2p250.
10
Biological concepts in virus classification.
Intervirology. 1988;29(5):242-53. doi: 10.1159/000150052.
引用本文的文献
1
Structural basis for the evolution of a domesticated group II intron-like reverse transcriptase to function in host cell DNA repair.一种驯化的II类内含子样逆转录酶在宿主细胞DNA修复中发挥功能的进化结构基础。
Proc Natl Acad Sci U S A. 2025 Aug 5;122(31):e2504208122. doi: 10.1073/pnas.2504208122. Epub 2025 Jul 29.
2
Structural basis for the evolution of a domesticated group II intron-like reverse transcriptase to function in host cell DNA repair.一种驯化的II类内含子样逆转录酶在宿主细胞DNA修复中发挥作用的进化的结构基础。
bioRxiv. 2025 Jan 15:2025.01.14.632616. doi: 10.1101/2025.01.14.632616.
3
Co-infection of Four Novel Mycoviruses from Three Lineages Confers Hypovirulence on Phytopathogenic Fungus Ustilaginoidea virens.来自三个谱系的四种新型真菌病毒的共感染赋予植物病原真菌稻曲病菌低毒力。
Rice (N Y). 2024 Jul 16;17(1):44. doi: 10.1186/s12284-024-00721-z.
4
Role of RNA Polymerase II Promoter-Proximal Pausing in Viral Transcription.RNA 聚合酶 II 启动子近端暂停在病毒转录中的作用。
Viruses. 2022 Sep 13;14(9):2029. doi: 10.3390/v14092029.
5
Group II intron-like reverse transcriptases function in double-strand break repair.II 组内含子样逆转录酶在双链断裂修复中发挥作用。
Cell. 2022 Sep 29;185(20):3671-3688.e23. doi: 10.1016/j.cell.2022.08.014. Epub 2022 Sep 15.
6
G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.G 蛋白亚基β1 通过促进 PB2 的核输入促进甲型流感病毒复制。
J Virol. 2022 Jun 22;96(12):e0049422. doi: 10.1128/jvi.00494-22. Epub 2022 May 23.
7
Bayesian Markov models improve the prediction of binding motifs beyond first order.贝叶斯马尔可夫模型超越一阶，改进了对结合基序的预测。
NAR Genom Bioinform. 2021 Apr 20;3(2):lqab026. doi: 10.1093/nargab/lqab026. eCollection 2021 Jun.
8
Eukaryotic Translation Elongation Factor 1 Delta Inhibits the Nuclear Import of the Nucleoprotein and PA-PB1 Heterodimer of Influenza A Virus.真核翻译延伸因子 1 德尔塔抑制甲型流感病毒核衣壳蛋白和 PA-PB1 异二聚体的核输入。
J Virol. 2020 Dec 22;95(2). doi: 10.1128/JVI.01391-20.
9
In silico study of chikungunya polymerase, a potential target for inhibitors.基孔肯雅病毒聚合酶的计算机模拟研究：抑制剂的潜在靶点
Virusdisease. 2019 Sep;30(3):394-402. doi: 10.1007/s13337-019-00547-0. Epub 2019 Oct 26.
10
The RNA Capping Enzyme Domain in Protein A is Essential for Flock House Virus Replication.蛋白 A 的 RNA 加帽酶结构域对于禽网状内皮组织增生病病毒的复制是必需的。
Viruses. 2018 Sep 9;10(9):483. doi: 10.3390/v10090483.
本文引用的文献
1
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.烟草叶绿体基因组的完整核苷酸序列：其基因组织与表达
EMBO J. 1986 Sep;5(9):2043-2049. doi: 10.1002/j.1460-2075.1986.tb04464.x.
2
Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.细胞色素b野生型和box3突变体中内含子及侧翼外显子的序列揭示了一个由内含子编码的交错剪接蛋白。
Cell. 1980 Nov;22(2 Pt 2):333-48. doi: 10.1016/0092-8674(80)90344-x.
3
Sequence and structure of yeast phosphoglycerate kinase.酵母磷酸甘油酸激酶的序列与结构
EMBO J. 1982;1(12):1635-40. doi: 10.1002/j.1460-2075.1982.tb01366.x.
4
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.大肠杆菌RNA聚合酶的一级结构。rpoB基因的核苷酸序列和β亚基的氨基酸序列。
Eur J Biochem. 1981 Jun 1;116(3):621-9. doi: 10.1111/j.1432-1033.1981.tb05381.x.
5
Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses.植物、动物和细菌病毒的RNA依赖性聚合酶的一级结构比较。
Nucleic Acids Res. 1984 Sep 25;12(18):7269-82. doi: 10.1093/nar/12.18.7269.
6
Structure of concanavalin A at 2.4-A resolution.伴刀豆球蛋白A在2.4埃分辨率下的结构。
Biochemistry. 1972 Dec 19;11(26):4910-9. doi: 10.1021/bi00776a006.
7
Structure of the GDP domain of EF-Tu and location of the amino acids homologous to ras oncogene proteins.EF-Tu的GDP结构域的结构以及与ras癌基因蛋白同源的氨基酸的位置。
Science. 1985 Oct 4;230(4721):32-6. doi: 10.1126/science.3898365.
8
Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.真核生物和原核生物RNA聚合酶最大亚基之间存在广泛的同源性。
Cell. 1985 Sep;42(2):599-610. doi: 10.1016/0092-8674(85)90117-5.
9
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.与脱氧胸苷一磷酸（dTMP）复合的大肠杆菌DNA聚合酶I大片段的结构
Nature. 1985;313(6005):762-6. doi: 10.1038/313762a0.
10
RNA polymerase. Direct evidence for two active sites involved in transcription.RNA聚合酶。转录过程中涉及两个活性位点的直接证据。
J Biol Chem. 1985 Feb 10;260(3):1427-31.
Zotero 插件