基于微流控技术生成用于细胞封装的尺寸可控、生物功能化的合成聚合物微凝胶。
Microfluidic-based generation of size-controlled, biofunctionalized synthetic polymer microgels for cell encapsulation.
作者信息
Headen Devon M, Aubry Guillaume, Lu Hang, García Andrés J
机构信息
Woodruff School of Mechanical Engineering, 315 Ferst Dr NW, Atlanta, GA, 30332, USA; Petit Institute for Bioengineering and Bioscience, 311 Ferst Dr NW, Atlanta, GA, 30332, USA.
出版信息
Adv Mater. 2014 May 21;26(19):3003-8. doi: 10.1002/adma.201304880. Epub 2014 Mar 11.
Abstract
Cell and islet microencapsulation in synthetic hydrogels provides an immunoprotective and cell-supportive microenvironment. A microfluidic strategy for the genaration of biofunctionalized, synthetic microgel particles with precise control over particle size and molecular permeability for cell and protein delivery is presented. These engineered capsules support high cell viability and function of encapsulated human stem cells and islets.
摘要
合成水凝胶中的细胞和胰岛微囊化提供了一个免疫保护和细胞支持的微环境。本文提出了一种微流控策略，用于生成生物功能化的合成微凝胶颗粒，可精确控制颗粒大小和分子渗透性，以用于细胞和蛋白质递送。这些工程化胶囊支持包封的人类干细胞和胰岛的高细胞活力和功能。
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Biomaterials. 2013 Nov;34(33):8122-30. doi: 10.1016/j.biomaterials.2013.07.079. Epub 2013 Aug 5.
2
Therapeutic cell encapsulation: ten steps towards clinical translation.治疗性细胞封装：迈向临床转化的十步。
J Control Release. 2013 Aug 28;170(1):1-14. doi: 10.1016/j.jconrel.2013.04.015. Epub 2013 May 1.
3
Metre-long cell-laden microfibres exhibit tissue morphologies and functions.长米级细胞填充微纤维展现出组织形态和功能。
Nat Mater. 2013 Jun;12(6):584-90. doi: 10.1038/nmat3606. Epub 2013 Mar 31.
4
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Biomaterials. 2013 Jun;34(19):4602-11. doi: 10.1016/j.biomaterials.2013.03.012. Epub 2013 Mar 26.
5
Microfluidic synthesis of cell-type-specific artificial extracellular matrix hydrogels.细胞类型特异性人工细胞外基质水凝胶的微流控合成。
Biomacromolecules. 2013 Apr 8;14(4):1122-31. doi: 10.1021/bm4000162. Epub 2013 Mar 8.
6
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Biomaterials. 2013 Apr;34(11):2683-93. doi: 10.1016/j.biomaterials.2013.01.015. Epub 2013 Jan 21.
7
A versatile approach to engineering biomolecule-presenting cellular microenvironments.一种构建呈现生物分子的细胞微环境的通用方法。
Adv Healthc Mater. 2013 Feb;2(2):292-6. doi: 10.1002/adhm.201200280. Epub 2012 Nov 7.
8
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9
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10
Microfluidic encapsulation of cells in polymer microgels.微流控法将细胞包封在聚合物微凝胶中。
Small. 2012 Jun 11;8(11):1633-42. doi: 10.1002/smll.201102464. Epub 2012 Mar 29.
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