Zhang Zhuqing, Bai Yan, Li Ping, Zhao Junjun, Wang Yanxia, Sun Lei, Tang Jianwu
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2013 Nov;27(22):1265-8.
To investigate the expression of signal transducer and activator of transcription 3 (STAT3) and phosphorylated STAT3 (p-STAT3) protein in papillary thyroid carcinoma (PTC), and to explore the correlation and significance between the expression of STAT3, p-STAT3 and epithelial-mesenchymal transition (EMT).
The expression of STATS3. p-STAT3, E-cadherin and Vimentin protein in 56 cases of PTC specimens and adjacent normal tissues specimens ware detected by immunohistochemistry. The correlation of the expression of STATS, p-STAT3, E-cadherin and Vimentin protein in PTC with clinicopathological characteristics was analyzed.
The positive rates of STAT3, p-STAT3 in PTC tissue were significantly higher than those in adjacent normal tissues specimens respectively (P < 0.01). The positive rates of E-cadherin in PTC tissues were remarkably lower, compared to adjacent normal tissues specimens (P < 0.01), however the positive rates of Vimentin in PTC tissues were remarkably higher, compared to adjacent normal tissues specimens (P < 0.01). The expression of STAT3, p-STAT3, E-cadherin and Vimentin protein were associated with lymph node metastasis and clinical stage (all P < 0.05). The expression of STAT3 and p-STAT3 was negatively correlated with E-cadherin expression (r = -0.494 and r = -0.364, P < 0.01, respectively), but positively with Vimentin expression (r = 0.533 and r = 0.377, P < 0.01, respectively) in PTC tissues.
PTC tissues have STAT3 protein activation and EMT phenotype, as were all correlated significantly with PTC invasion and metastasis. STAT3 signaling pathway activation might mediate EMT and then promote PTC invasion and metastasis.
探讨信号转导与转录激活因子3（STAT3）及磷酸化STAT3（p-STAT3）蛋白在甲状腺乳头状癌（PTC）中的表达情况，以及STAT3、p-STAT3表达与上皮-间质转化（EMT）之间的相关性及意义。
采用免疫组织化学法检测56例PTC标本及癌旁正常组织标本中STAT3、p-STAT3、E-钙黏蛋白和波形蛋白的表达。分析PTC中STAT3、p-STAT3、E-钙黏蛋白和波形蛋白表达与临床病理特征的相关性。
PTC组织中STAT3、p-STAT3的阳性率分别显著高于癌旁正常组织标本（P<0.01）。PTC组织中E-钙黏蛋白的阳性率明显低于癌旁正常组织标本（P<0.01），而波形蛋白的阳性率明显高于癌旁正常组织标本（P<0.01）。STAT3、p-STAT3、E-钙黏蛋白和波形蛋白的表达与淋巴结转移及临床分期相关（均P<0.05）。PTC组织中STAT3和p-STAT3的表达与E-钙黏蛋白的表达呈负相关（r分别为-0.494和-0.364，P均<0.01），但与波形蛋白的表达呈正相关（r分别为0.533和0.377，P均<0.01）。
PTC组织存在STAT3蛋白激活及EMT表型，且均与PTC的侵袭和转移显著相关。STAT3信号通路激活可能介导EMT，进而促进PTC的侵袭和转移。
