Environ Technol. 2013 Nov-Dec;34(21-24):3069-77. doi: 10.1080/09593330.2013.803131.
Fluorescence spectroscopy was used to characterize municipal wastewater at various stages of treatment in order to understand how its fluorescence signature changes with treatment and how the signal relates to biochemical oxygen demand (BOD) and chemical oxygen demand (COD). The impact of size fractionation on the fluorescence signal was also investigated. Fluorescence measurements were taken for unfiltered and filtered (0.45 and 0.20 microm) samples of crude, settled and secondary treated wastewater (activated sludge and trickling filter), and final effluent. Good correlations were observed for unfiltered, diluted wastewater samples between BOD and fluorescence intensity at excitation 280 nm, emission 350 nm (Peak T1) (r = 0.92) and between COD and Peak T1 intensity (r = 0.85). The majority of the T1 and T2 signal was found to be derived from the <0.20 microm fraction. Initial results indicate that fluorescence spectroscopy, and changes in Peak T1 intensity in particular, could be used for continuous, real-time wastewater quality assessment and process control of wastewater treatment works.
荧光光谱法用于表征不同处理阶段的城市废水，以了解其荧光特征如何随处理而变化，以及信号如何与生化需氧量（BOD）和化学需氧量（COD）相关。还研究了大小分级对荧光信号的影响。对未经滤和过滤（0.45 和 0.20 微米）的原水、沉降和二级处理（活性污泥和滴滤池）废水以及最终出水进行了荧光测量。对于未经滤、稀释的废水样品，在激发 280nm、发射 350nm（峰 T1）（r=0.92）处 BOD 和荧光强度之间以及 COD 和峰 T1 强度之间（r=0.85）观察到良好的相关性。T1 和 T2 信号的大部分源自<0.20 微米级分。初步结果表明，荧光光谱法，特别是峰 T1 强度的变化，可用于连续、实时的废水质量评估和废水处理厂的过程控制。
