Yan Yaping, Li Zhiying, Dong Minghua, Zhou Qiao, Jin Fangyou, Yang Liyuan, Li Shaolan
Wei Sheng Wu Xue Bao. 2013 Nov 4;53(11):1205-12.
The aims of this study were to investigate yeast diversity in Yangzonghai Lake, and to explore the value of these yeasts in such ecological environment, and at the same time, to analyze biological factors and abiotic factors on the influence of the yeast diversity.
Water was filtered through cellulose acetate membrane, serial dilutions of soil and sediment; The D1/D2 domain of 26S rRNA gene of all yeast strains have been sequenced and analyzed. The ability of the yeast strains to produce various enzymes was tested by enzyme screening plates.
In total of 201 yeast strains were isolated,these strains were identified as 48 species in 15 genera, including 10 presumed new species or variety; 15.9% showed at least one extracellular enzymatic activity, mainly the lipases and amylases.
The yeast community from Yangzonghai Lake showed high species richness, and anthropogenical influenced is an important factor for the yeast count and distribution, at same time, conductivity and turbidity are also important factors for yeast distribution; Some yeasts produced at least one extracellular enzymatic activity, which suggested that these microorganisms are participating in nature cycle and are metabolically active in the lake.
本研究旨在调查阳宗海湖中的酵母多样性，探索这些酵母在该生态环境中的价值，同时分析生物因素和非生物因素对酵母多样性的影响。
通过醋酸纤维素膜过滤湖水，对土壤和沉积物进行系列稀释；对所有酵母菌株的26S rRNA基因的D1/D2结构域进行测序和分析。通过酶筛选平板测试酵母菌株产生各种酶的能力。
共分离出201株酵母菌株，这些菌株被鉴定为15个属的48个种，包括10个假定的新物种或变种；15.9%的菌株表现出至少一种细胞外酶活性，主要是脂肪酶和淀粉酶。
阳宗海湖的酵母群落显示出较高的物种丰富度，人为影响是酵母数量和分布的重要因素，同时，电导率和浊度也是酵母分布的重要因素；一些酵母产生了至少一种细胞外酶活性，这表明这些微生物参与了自然循环，并且在湖中具有代谢活性。
