Mokhtarnejad Lachin, Arzanlou Mahdi, Babai-Ahari Asadollah, Di Mauro Simone, Onofri Andrea, Buzzini Pietro, Turchetti Benedetta
Plant Protection Department, Agriculture Faculty, University of Tabriz, P.O. Box: 5166614766, Tabriz, Iran.
Department of Agricultural, Environmental and Food Sciences & Industrial Yeasts Collection DBVPG, University of Perugia, Perugia, Italy.
Extremophiles. 2016 Nov;20(6):915-928. doi: 10.1007/s00792-016-0883-1. Epub 2016 Oct 21.
Urmia Lake, located in northwest Iran, is an oligotrophic and extremely hypersaline habitat that supports diverse forms of life. Owing to its unique biodiversity and special environmental conditions, Urmia Lake National Park has been designated as one of the biosphere reserves by UNESCO. This study was aimed to characterize basidiomycetous yeasts in hypersaline soils surrounding the Urmia Lake National Park using a polyphasic combination of molecular and physiological data. Soil samples were collected from eight sites in Lake Basin and six islands insides the lake. Yeast strains were identified by sequencing the D1/D2 domains of the 26S rRNA gene. When D1/D2 domain sequencing did not resolve the identity of the species, strain identification was obtained by ITS 1 & 2 sequencing. Twenty-one species belonging to the genera Cystobasidium, Holtermanniella, Naganishia, Rhodotorula, Saitozyma, Solicoccozyma, Tausonia, Vanrija, and Vishniacozyma were identified. Solicoccozyma aeria represented the dominant species. The ability of isolates to grow at 10 and 15 % of NaCl was checked; about two-thirds of the strains grew at 10 %, while about 13 % of the isolates grew in medium with 15 % NaCl. this study is the first study on the culturable yeast diversity in hypersaline soils surrounding an Asian lake.
乌尔米耶湖位于伊朗西北部，是一个贫营养且盐度极高的栖息地，拥有多样的生命形式。由于其独特的生物多样性和特殊的环境条件，乌尔米耶湖国家公园已被联合国教科文组织指定为生物圈保护区之一。本研究旨在利用分子和生理数据的多相组合，对乌尔米耶湖国家公园周边高盐土壤中的担子菌酵母进行特征描述。从湖盆的八个地点和湖内的六个岛屿采集了土壤样本。通过对26S rRNA基因的D1/D2结构域进行测序来鉴定酵母菌株。当D1/D2结构域测序无法确定物种身份时，通过ITS 1和2测序来进行菌株鉴定。鉴定出了属于囊担子菌属、霍尔特曼酵母属、纳加尼西亚酵母属、红酵母属、斋藤酵母属、索利科酵母属、陶索尼酵母属、万氏酵母属和维什尼亚酵母属的21个物种。气生索利科酵母是优势物种。检测了分离株在10%和15%氯化钠浓度下生长的能力；约三分之二的菌株能在10%氯化钠浓度下生长，而约13%的分离株能在含15%氯化钠的培养基中生长。本研究是关于亚洲湖泊周边高盐土壤中可培养酵母多样性的首次研究。
