沙特阿拉伯利雅得女性宫颈标本中的人乳头瘤病毒：一项基于医院的研究。

Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based study.

作者信息

Al-Ahdal Mohammed N, Al-Arnous Walaa K, Bohol Marie F, Abuzaid Suhair M, Shoukri Mohamed M, Elrady Kamal S, Firdous Nayyer, Aliyan Rehab, Taseer Rabia, Al-Hazzani Amal A, Al-Qahtani Ahmed A

机构信息

King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.

出版信息

J Infect Dev Ctries. 2014 Mar 13;8(3):320-5. doi: 10.3855/jidc.4220.

DOI:10.3855/jidc.4220

PMID:24619263

Abstract

INTRODUCTION

Certain genotypes of human papillomavirus (HPV) are linked to cervical abnormalities. HPV DNA and genotype prevalence among women residing in Riyadh, Saudi Arabia is investigated in this hospital-based study.

METHODOLOGY

Cervical specimens were taken from 519 subjects along with consent and demographic data. DNA was extracted and PCR was performed on all specimens using general primers. Low- and high-risk HPV genotypes were determined by reverse blot hybridization assay using specific probes. SPSS version 17 was used for the data analysis.

RESULTS

Of 519 cervical specimens, 164 (31.6%) were positive for HPV DNA. There was a significant association between HPV positivity and abnormal cytology (p < 0.00001). Even though the HPV positivity was relatively high, the squamous intraepithelial lesions were minimal, with one low grade and one high grade case among those HPV DNA-positive specimens. Regardless of single or multiple infections per specimen, HPV-16 was found in 87.8%, followed by HPV-18 in 86%, and HPV-11 in 78.3%.

CONCLUSIONS

Amplification technology showed that HPV is common among women in Riyadh, Saudi Arabia, with a strong association between HPV infection and cytological changes. HPV-16 was the most frequent genotype but had a low prevalence of cervical cancer.

摘要

引言

某些人乳头瘤病毒（HPV）基因型与宫颈异常有关。在这项基于医院的研究中，对居住在沙特阿拉伯利雅得的女性进行了HPV DNA和基因型患病率调查。

方法

在征得同意并收集人口统计学数据的情况下，从519名受试者中采集宫颈样本。提取DNA，并使用通用引物对所有样本进行聚合酶链反应（PCR）。使用特异性探针通过反向斑点杂交试验确定低风险和高风险HPV基因型。使用SPSS 17版进行数据分析。

结果

在519份宫颈样本中，164份（31.6%）HPV DNA呈阳性。HPV阳性与细胞学异常之间存在显著关联（p < 0.00001）。尽管HPV阳性率相对较高，但鳞状上皮内病变极少，在那些HPV DNA阳性样本中仅有1例低级别和例高级别病例。无论每个样本是单一感染还是多重感染，HPV-16的检出率为87.8%，其次是HPV-18，为86%，HPV-11为78.3%。