Department of Biomedical Sciences and Morphological and Functional Images, University of Messina, Messina, Italy.
Br J Haematol. 2014 Jun;165(6):801-13. doi: 10.1111/bjh.12828. Epub 2014 Mar 12.
MicroRNAs (miRNAs) are short non-coding RNAs involved in the regulation of gene expression. Selected groups of miRNAs are differentially expressed in various types of cancers. Alterations in miRNAs gene expression have been shown in cells from the B-cell malignancy, multiple myeloma (MM). However, although MM is a disease of plasma cells, abnormalities have been detected in the peripheral blood of the patients. The goal of our study was to analyse the entire miRNome in peripheral lymphocytes of MM patients using reverse transcription quantitative polymerase chain reaction. Using in silica analysis, we also evaluated some of the most interesting and significant pathways. Analysis revealed that MM samples had a distinct miRNA profile compared to the controls. This resulted in the identification of 203 miRNAs, 85 of which were over-expressed and 118 under-expressed. Of these, 184 possessed validated or highly predicted mRNA targets. We identified 12 354 mRNA targets of the transcriptome: 36·4% of the related proteins are involved in death processes while the 21% are required for growth and cell proliferation. We have demonstrated that miRNAs are differentially expressed in the peripheral blood of MM patients compared to controls, affecting some pathways involved in the anti-apoptotic process, cell proliferation and maybe anti-angiogenesis.
微小 RNA（miRNAs）是参与基因表达调控的短非编码 RNA。在各种类型的癌症中，特定的 miRNA 组表现出差异表达。miRNAs 基因表达的改变已在 B 细胞恶性肿瘤多发性骨髓瘤（MM）的细胞中被发现。然而，尽管 MM 是一种浆细胞疾病，但在患者的外周血中已经检测到异常。我们的研究目的是使用逆转录定量聚合酶链反应分析 MM 患者外周淋巴细胞中的整个 miRNA 组。通过计算机分析，我们还评估了一些最有趣和最重要的途径。分析表明，与对照组相比，MM 样本具有独特的 miRNA 谱。这导致鉴定了 203 个 miRNA，其中 85 个表达上调，118 个表达下调。其中，184 个具有验证或高度预测的 mRNA 靶标。我们鉴定了转录组的 12354 个 mRNA 靶标：相关蛋白的 36.4% 参与死亡过程，而 21% 则需要生长和细胞增殖。我们已经证明，与对照组相比，MM 患者外周血中的 miRNAs 表达存在差异，影响一些参与抗凋亡过程、细胞增殖和可能抗血管生成的途径。
