Wijaya Wisiani, Pang Shintaro, Labuza Theodore P, He Lili
Dept. of Food Science, Univ. of Massachusetts Amherst, 100 Holdsworth Way, Amherst, MA, 01003, USA.
J Food Sci. 2014 Apr;79(4):T743-7. doi: 10.1111/1750-3841.12391. Epub 2014 Mar 12.
Acetamiprid is a neonicotinoid pesticide that is commonly used in modern farming. Acetamiprid residue in food commodities can be a potential harm to human and has been implicated in the honey bee hive die off crisis. In this study, we developed rapid, simple, and sensitive methods to detect acetamiprid in apple juice and on apple surfaces using surface-enhanced Raman spectroscopy (SERS). No pretreatment of apple juice sample was performed. A simple surface swab method was used to recover acetamiprid from the apple surface. Samples were incubated with silver dendrites for several minutes and SERS spectra were taken directly from the silver surface. Detection of a set of 5 apple juice samples can be done within 10 min. The swab-SERS method took 15 min for a set of 5 samples. Resulting spectral data were analyzed using principal component analysis. The highest acetamiprid peak at 634 cm(-1) was used to detect and quantify the amount of acetamiprid spiked in 1:1 water-methanol solvent, apple juice, and on apple surface. The SERS method was able to successfully detect acetamiprid at 0.5 μg/mL (0.5 ppm) in solvent, 3 μg/mL (3 ppm) in apple juice, and 0.125 μg/cm(2) on apple surfaces. The SERS methods provide simple, rapid, and sensitive ways to detect acetamiprid in beverages and on the surfaces of thick skinned fruits and vegetables.
啶虫脒是一种常用于现代农业的新烟碱类杀虫剂。食品中的啶虫脒残留可能对人体造成潜在危害，并且与蜜蜂蜂群死亡危机有关。在本研究中，我们开发了快速、简单且灵敏的方法，利用表面增强拉曼光谱（SERS）检测苹果汁和苹果表面的啶虫脒。苹果汁样品无需预处理。采用简单的表面擦拭方法从苹果表面回收啶虫脒。样品与银树枝晶孵育几分钟后，直接从银表面获取SERS光谱。一组5个苹果汁样品的检测可在10分钟内完成。对于一组5个样品，擦拭 - SERS方法需要15分钟。使用主成分分析对所得光谱数据进行分析。在634 cm(-1)处的最高啶虫脒峰用于检测和定量添加到1:1水 - 甲醇溶剂、苹果汁以及苹果表面的啶虫脒含量。SERS方法能够成功检测出溶剂中0.5 μg/mL（0.5 ppm）、苹果汁中3 μg/mL（3 ppm）以及苹果表面0.125 μg/cm(2)的啶虫脒。SERS方法提供了简单、快速且灵敏的方式来检测饮料以及厚皮水果和蔬菜表面的啶虫脒。
