Du Shuyuan, Yu Chundi, Tang Lin, Lu Lixia
Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, China.
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Nanomaterials (Basel). 2018 Sep 25;8(10):757. doi: 10.3390/nano8100757.
A wide variety of biotic and abiotic stresses continually attack plants and animals, which adversely affect their growth, development, reproduction, and yield realization. To survive under stress conditions, highly sophisticated and efficient tolerance mechanisms have been evolved to adapt to stresses, which consist of the variation of effector molecules playing vital roles in physiological regulation. The development of a sensitive, facile, and rapid analytical methods for stress factors and effector molecules detection is significant for gaining deeper insight into the tolerance mechanisms. As a nondestructive analysis technique, surface-enhanced Raman spectroscopy (SERS) has unique advantages regarding its biosensing applications. It not only provides specific fingerprint spectra of the target molecules, conformation, and structure, but also has universal capacity for simultaneous detection and imaging of targets owing to the narrow width of the Raman vibrational bands. Herein, recent progress on biotic and abiotic stresses, tolerance mechanisms and effector molecules is summarized. Moreover, the development and promising future trends of SERS detection for stress-related substances combined with nanomaterials as substrates and SERS tags are discussed. This comprehensive and critical review might shed light on a new perspective for SERS applications.
各种各样的生物和非生物胁迫持续侵袭动植物，对它们的生长、发育、繁殖及产量实现产生不利影响。为了在胁迫条件下生存，植物和动物进化出了高度复杂且高效的耐受机制来适应胁迫，这些机制包括在生理调节中起关键作用的效应分子的变化。开发用于检测胁迫因子和效应分子的灵敏、简便且快速的分析方法，对于深入了解耐受机制具有重要意义。作为一种无损分析技术，表面增强拉曼光谱（SERS）在生物传感应用方面具有独特优势。它不仅能提供目标分子的特定指纹光谱、构象和结构，而且由于拉曼振动带宽度窄，还具有同时检测和成像目标的通用能力。本文总结了生物和非生物胁迫、耐受机制及效应分子方面的最新进展。此外，还讨论了以纳米材料为底物和SERS标签的SERS检测胁迫相关物质的发展情况及未来的发展趋势。这篇全面且具批判性的综述可能会为SERS应用提供新的视角。
