Grups J W, Frohmüller H G
Department of Urology, University of Würzburg, Medical School, FRG.
Urol Int. 1988;43(5):265-8. doi: 10.1159/000281351.
In order to evaluate the antiproliferative effects of recombinant human interferon-gamma 2c (rHu IFN-gamma 2c), recombinant human interferon-gamma (rHu IFN-gamma), natural interferon-beta (IFN-beta), and their combination with cytotoxic agents, 17 different human bladder carcinoma cell lines were tested in vitro. The antiproliferative effects were compared in evaluating the tumor cell inhibiting potency of the different interferon (IFN) classes. It could be demonstrated that interferons have inhibiting effects on the bladder cancer cell multiplication rate, yet there are significant differences in the susceptibility of different IFN preparations on different cell lines. The cell lines BT1, RT4, EJ, 468P, 253J, SD, TCCSUP, and SW1738 can be defined as sensitive, T24, 647V, VM-CUB2, and J82 as semisensitive, HT1376, 5637, VM-CUB1, 639V and SW1710 as resistant upon treatment with IFN. The combination of rHu IFN-alpha 2c, IFN-beta, and rHu IFN-gamma seems to be more effective than treatment with rHu IFN-alpha 2c alone. The cytotoxic effect of doxorubicin on bladder cancer cells can be intensified by combining it with IFN.
为了评估重组人干扰素-γ2c（rHu IFN-γ2c）、重组人干扰素-γ（rHu IFN-γ）、天然干扰素-β（IFN-β）及其与细胞毒性药物联合使用的抗增殖作用，对17种不同的人膀胱癌细胞系进行了体外测试。在评估不同类别干扰素（IFN）的肿瘤细胞抑制效力时，比较了它们的抗增殖作用。结果表明，干扰素对膀胱癌细胞的增殖率有抑制作用，但不同的IFN制剂对不同细胞系的敏感性存在显著差异。经IFN处理后，BT1、RT4、EJ、468P、253J、SD、TCCSUP和SW1738细胞系可定义为敏感，T24、647V、VM-CUB2和J82为半敏感，HT1376、5637、VM-CUB1、639V和SW1710为耐药。rHu IFN-α2c、IFN-β和rHu IFN-γ联合使用似乎比单独使用rHu IFN-α2c更有效。阿霉素与IFN联合使用可增强其对膀胱癌细胞的细胞毒性作用。
