Borden E C, Groveman D S, Nasu T, Reznikoff C, Bryan G T
J Urol. 1984 Oct;132(4):800-3. doi: 10.1016/s0022-5347(17)49877-6.
The antiproliferative effect of interferons against 5 human bladder carcinoma cell lines, RT112, T24, RT4, 647V and HT1197, was determined in vitro. Each of these human bladder carcinoma cell lines except 647V was sensitive to human interferons in liquid media. The antiproliferative effect of interferons was observed only upon continuous exposure, not after 1 hour. Partially purified, naturally produced interferon beta was more inhibitory of cell growth than naturally produced interferon alpha. Interferon alpha 54, 76, 61, 6L and 1 purified to homogeneity were as effective as naturally produced, partially pure interferon alpha. Although interferon beta, produced by recombinant DNA technology and purified to homogeneity, was not equivalent in effectiveness to naturally produced interferon beta, its antiproliferative activity was greater than interferon alpha 54 for 3 of 4 cell lines tested. Antimitotic effects may underlie, at least in part, the potential therapeutic activity of interferons for bladder carcinoma.
在体外测定了干扰素对5种人膀胱癌细胞系RT112、T24、RT4、647V和HT1197的抗增殖作用。除647V外，这些人膀胱癌细胞系中的每一种在液体培养基中对人干扰素均敏感。干扰素的抗增殖作用仅在持续暴露时观察到，而非在1小时后。部分纯化的天然产生的干扰素β比天然产生的干扰素α对细胞生长的抑制作用更强。纯化至均一性的干扰素α54、76、61、6L和1与天然产生的部分纯化的干扰素α效果相同。虽然通过重组DNA技术产生并纯化至均一性的干扰素β在有效性上与天然产生的干扰素β不等同，但其对所测试的4种细胞系中的3种的抗增殖活性大于干扰素α54。抗有丝分裂作用可能至少部分地是干扰素对膀胱癌潜在治疗活性的基础。
