表面等离子体共振技术在神经退行性疾病发病机制及治疗中重要分子表征方面的应用
Applications of SPR for the characterization of molecules important in the pathogenesis and treatment of neurodegenerative diseases.
作者信息
Wittenberg Nathan J, Wootla Bharath, Jordan Luke R, Denic Aleksandar, Warrington Arthur E, Oh Sang-Hyun, Rodriguez Moses
机构信息
Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, USA.
出版信息
Expert Rev Neurother. 2014 Apr;14(4):449-63. doi: 10.1586/14737175.2014.896199.
Characterization of binding kinetics and affinity between a potential drug and its receptor are key steps in the development of new drugs. Among the techniques available to determine binding affinities, surface plasmon resonance has emerged as the gold standard because it can measure binding and dissociation rates in real-time in a label-free fashion. Surface plasmon resonance is now finding applications in the characterization of molecules for treatment of neurodegenerative diseases, characterization of molecules associated with pathogenesis of neurodegenerative diseases and detection of neurodegenerative disease biomarkers. In addition it has been used in the characterization of a new class of natural autoantibodies that have therapeutic potential in a number of neurologic diseases. In this review we will introduce surface plasmon resonance and describe some applications of the technique that pertain to neurodegenerative disorders and their treatment.
表征潜在药物与其受体之间的结合动力学和亲和力是新药研发的关键步骤。在可用于测定结合亲和力的技术中，表面等离子体共振已成为金标准，因为它能够以无标记方式实时测量结合和解离速率。表面等离子体共振目前正应用于神经退行性疾病治疗分子的表征、与神经退行性疾病发病机制相关分子的表征以及神经退行性疾病生物标志物的检测。此外，它还被用于表征一类新型天然自身抗体，这类抗体在多种神经系统疾病中具有治疗潜力。在本综述中，我们将介绍表面等离子体共振，并描述该技术在神经退行性疾病及其治疗方面的一些应用。
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