乌干达艾滋病诊所中从患有口腔念珠菌病的人类免疫缺陷病毒感染者体内分离出的念珠菌属的细胞外水解酶活性和生物膜形成情况
Extracellular hydrolytic enzyme activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus with oropharyngeal candidiasis at HIV/AIDS clinics in Uganda.
作者信息
Musinguzi Benson, Akampurira Andrew, Derick Hope, Turyamuhika Laban, Mwesigwa Alex, Mwebesa Edson, Mwesigye Vicent, Kabajulizi Immaculate, Sekulima Tahalu, Ocheng Francis, Itabangi Herbert, Mboowa Gerald, Sande Obondo James, Achan Beatrice
机构信息
Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda; Department of Medical Laboratory Sciences, Faculty of Health Sciences, Muni University, P.O. Box 725, Arua, Uganda.
Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, P.O. Box 7072, Makerere University, Kampala, Uganda.
出版信息
Microb Pathog. 2025 Feb;199:107232. doi: 10.1016/j.micpath.2024.107232. Epub 2024 Dec 14.
BACKGROUND
Commensal oral Candida species can become opportunistic and transition to pathogenic causes of oropharyngeal candidiasis (OPC) in individuals with impaired immunity through ecological cues and the expression of extracellular hydrolytic enzyme activities and biofilm formation.
OBJECTIVE
We evaluated phospholipase, proteinase, hemolysin, esterase, and coagulase enzymatic activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus (PLHIV) with OPC.
METHODS
Thirty-five Candida isolates from PLHIV with OPC were retrieved from a sample repository and evaluated for phospholipase activity using the egg yolk agar method, proteinase activity using the bovine serum albumin agar method, hemolysin activity using the blood agar plate method, esterase activity using the Tween 80 opacity test medium method, coagulase activity using the classical tube method, and biofilm formation using the microtiter plate assay method in vitro.
RESULTS
A total of 35 Candida isolates obtained from PLHIV with OPC were included in this study, and phospholipase and proteinase activities were detected in 33/35 (94.3 %) and 31/35 (88.6 %) Candida isolates, respectively. Up to 25/35 (71.4 %) of the Candida isolates exhibited biofilm formation, whereas esterase activity was demonstrated in 23/35 (65.7 %) of the Candida isolates. Fewer isolates (21/35, 60 %) produced hemolysin, and coagulase production was the least common virulence activity detected in 18/35 (51.4 %) of the Candida isolates.
CONCLUSION
Phospholipase and proteinase activities were the strongest in oropharyngeal Candida species.
背景
共生口腔念珠菌可通过生态线索、细胞外水解酶活性的表达和生物膜形成，在免疫力受损个体中转变为机会性致病菌，引发口腔念珠菌病（OPC）。
目的
我们评估了从患有OPC的人类免疫缺陷病毒（PLHIV）感染者中分离出的念珠菌的磷脂酶、蛋白酶、溶血素、酯酶和凝固酶的酶活性以及生物膜形成情况。
方法
从样本库中获取35株来自患有OPC的PLHIV感染者的念珠菌分离株，采用蛋黄琼脂法评估磷脂酶活性，采用牛血清白蛋白琼脂法评估蛋白酶活性，采用血琼脂平板法评估溶血素活性，采用吐温80不透明度测试培养基法评估酯酶活性，采用经典试管法评估凝固酶活性，并在体外采用微量滴定板测定法评估生物膜形成情况。
结果
本研究共纳入35株从患有OPC的PLHIV感染者中获得的念珠菌分离株，分别在33/35（94.3%）和31/35（88.6%）的念珠菌分离株中检测到磷脂酶和蛋白酶活性。高达25/35（71.4%）的念珠菌分离株表现出生物膜形成，而23/35（65.7%）的念珠菌分离株显示有酯酶活性。较少的分离株（21/35，60%）产生溶血素，凝固酶产生是在18/35（51.4%）的念珠菌分离株中检测到的最不常见的毒力活性。
结论
磷脂酶和蛋白酶活性在口腔念珠菌中最强。
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