Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou & Guizhou Talent Base for Microbiology and Human Health, Key Laboratory of Medical Microbiology and Parasitology of Education Department of Guizhou, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.
Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
Yeast. 2019 May;36(5):363-373. doi: 10.1002/yea.3397.
A set of 185 strains of Candida albicans from patients with vulvovaginal candidiasis (VVC) and from non-VVC clinical sources in southwest China was analysed. Strains were subjected to genotyping using CAI microsatellite typing and amplification of an intron-containing region of the 25S rRNA gene. Microsatellite genotypes of strains from non-VVC sources showed high polymorphism, whereas those of VVC were dominated by few, closely similar genotypes. However, among non-VVC strains, two genotypes were particularly prevalent in patients with lung cancer. 25S rDNA genotype A was dominant in VVC sources (86.7%), whereas genotypes A, B, and C were rather evenly distributed among non-VVC sources; known genotypes D and E were not found. In an experimental mouse model, isolates from lung cancer and AIDS patients proved to have higher virulence than VVC strains. Among 156 mice infected with C. albicans, 19 developed non-invasive urothelial carcinoma. No correlation could be established between parameters of virulence, source of infection, and incidence of carcinoma. C. albicans strains from VVC were less susceptible to itraconazole than the strains from non-VVC sources, whereas there was small difference in antifungal susceptibility between different 25S rDNA genotypes of C. albicans tested against amphotericin B, itraconazole, fluconazole, and flucytosine.
从中国西南部患有外阴阴道念珠菌病（VVC）的患者和非 VVC 临床来源的 185 株白色念珠菌进行了分析。使用 CAI 微卫星分型和内含子扩增对 25S rRNA 基因的菌株进行基因分型。非 VVC 来源的菌株的微卫星基因型表现出高度多态性，而 VVC 的菌株则由少数密切相似的基因型主导。然而，在非 VVC 菌株中，两种基因型在肺癌患者中特别流行。25S rDNA 基因型 A 在 VVC 来源中占优势（86.7%），而基因型 A、B 和 C 在非 VVC 来源中分布均匀；未发现已知的基因型 D 和 E。在实验性小鼠模型中，来自肺癌和艾滋病患者的分离株被证明比 VVC 菌株具有更高的毒力。在感染了白色念珠菌的 156 只小鼠中，有 19 只发展为非侵袭性尿路上皮癌。毒力参数、感染来源和癌的发生率之间没有相关性。与非 VVC 来源的菌株相比，来自 VVC 的白色念珠菌菌株对伊曲康唑的敏感性较低，而对两性霉素 B、伊曲康唑、氟康唑和氟胞嘧啶进行测试的不同 25S rDNA 基因型的白色念珠菌之间的抗真菌敏感性差异很小。
