Yaksh T L
Laboratory of Neurosurgical Research, Mayo Clinic, Rochester, MN 55905.
Brain Res. 1988 Aug 23;458(2):319-24. doi: 10.1016/0006-8993(88)90474-x.
Perfusate obtained from the intra-articular space of the knee (100 microliters/min) in the halothane-anesthetized cat under resting conditions shows no detectable substance P-like immunoreactivity (SP-LI: less than 2 pg/20 min). Antidromic activation by electrical stimulation of the sciatic nerve at stimulus intensities which evoke activity in A beta/A delta/C but not A beta fibers or the local administration of capsaicin (10(-4) M) into the intra-articular perfusate results in a stimulus-dependent increase in the levels of SP-LI in the perfusate. This release is diminished in a naloxone-(1 mg/kg, i.v.) reversible fashion by sufentanil, D-Ala2-D-Leu5-enkephalin, and D-Pen2-D-Pen5-enkephalin, but not by U50488H (10(-4) M) added to the intra-articular perfusate. These observations thus suggest that the peripheral stores of SP-LI in small primary afferents are subject to release by antidromic activity and this release is subject to modulation by mu/delta receptors on these peripheral terminals.
在氟烷麻醉的猫处于静息状态下，从膝关节腔内获取的灌流液（100微升/分钟）未显示出可检测到的P物质样免疫反应性（P物质样免疫反应性：低于2皮克/20分钟）。以能诱发Aβ/Aδ/C纤维而非Aβ纤维活动的刺激强度对坐骨神经进行电刺激逆行激活，或向关节内灌流液中局部施用辣椒素（10⁻⁴ M），会导致灌流液中P物质样免疫反应性水平出现刺激依赖性升高。这种释放可被舒芬太尼、D - 丙氨酸² - D - 亮氨酸⁵ - 脑啡肽和D - 青霉胺² - D - 青霉胺⁵ - 脑啡肽以纳洛酮（1毫克/千克，静脉注射）可逆的方式减弱，但向关节内灌流液中添加U50488H（10⁻⁴ M）则不会。因此，这些观察结果表明，小的初级传入神经中P物质样免疫反应性的外周储存可通过逆行活动释放，且这种释放受到这些外周终末上的μ/δ受体的调节。
