Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., České Budějovice, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic.
Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic.
Vet Parasitol. 2014 May 28;202(3-4):330-4. doi: 10.1016/j.vetpar.2014.02.012. Epub 2014 Feb 18.
Piglets from 4 to 8 weeks of age originated from a Cryptosporidium-free research breed were orally inoculated with 1 × 10(6) infectious oocysts of Cryptosporidium scrofarum. The number of shed oocysts per gram of faeces served to describe the infection intensity and prepatent period. In addition, faecal samples collected daily and tissue samples of the small and large intestine collected at 30 days post-inoculation were examined for the C. scrofarum small subunit ribosomal RNA gene using PCR. The piglets inoculated at 4-weeks of age remained uninfected, whereas 5-week-old and older animals were fully susceptible with a prepatent period ranging from 4 to 8 days. Susceptible pigs shed oocysts intermittently, and shedding intensity, reaching a mean maximum of 6000 oocysts per gram, did not differ significantly among infected animals. This study demonstrates that pigs become susceptible to C. scrofarum infection as late as 5-weeks of age. The mechanisms of age related susceptibility remain unknown.
4 至 8 周龄的仔猪来自无隐孢子虫的研究品种，经口接种 1×10(6)个感染性隐孢子虫卵囊。每克粪便中脱落的卵囊数用于描述感染强度和潜伏期。此外，在接种后 30 天收集每日粪便样本和小肠和大肠组织样本，使用 PCR 检测隐孢子虫小亚基核糖体 RNA 基因。4 周龄接种的仔猪未感染，而 5 周龄及以上的动物完全易感，潜伏期为 4 至 8 天。易感猪间歇性排出卵囊，感染动物的排出强度达到平均最大值 6000 个卵囊/克，没有显著差异。本研究表明，猪在 5 周龄时才易感染隐孢子虫。年龄相关易感性的机制尚不清楚。
