Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, vvi, České Budějovice, Czech Republic.
Exp Parasitol. 2012 May;131(1):107-10. doi: 10.1016/j.exppara.2012.03.016. Epub 2012 Mar 23.
Three and 8 week old pigs were inoculated with Cryptosporidium muris HZ206 (Mus musculus musculus isolate), Cryptosporidium tyzerri CR2090 (M. m. musculus isolate) or C. tyzzeri CR4293 (isolate from a hybrid between Mus musculus domesticus and M. m. musculus) at a dose of 1 × 10(7) oocysts per animal. Inoculated pigs showed no detectable infection and no clinical symptoms of cryptosporidiosis during 30 days post infection (DPI), and no macroscopic changes were detected in the digestive tract following necropsy. Developmental stages were not detected in gastrointestinal tract tissue by histology or PCR throughout the duration of the experiment. The infectivity of isolates was verified on SCID mice, in which oocysts shedding started from 4 to 8 DPI. Based on our findings, it can be concluded that pigs are not susceptible to C. muris or C. tyzzeri infection.
将 3 周和 8 周龄的猪用 1×10(7)个卵囊/只的剂量接种 Cryptosporidium muris HZ206（Mus musculus musculus 分离株）、Cryptosporidium tyzerri CR2090（M. m. musculus 分离株）或 C. tyzzeri CR4293（家猪和 M. m. musculus 之间的杂交种的分离株）。接种猪在感染后 30 天（DPI）内未显示出可检测的感染和隐孢子虫病的临床症状，剖检后消化道也未发现宏观变化。在整个实验过程中，通过组织学或 PCR 均未在胃肠道组织中检测到发育阶段。分离株的感染力在 SCID 小鼠中得到验证，其中卵囊脱落从 4 至 8 DPI 开始。根据我们的发现，可以得出结论，猪不易感染 C. muris 或 C. tyzzeri 感染。
