School of Bioscience and Bioengineering, South China University of Technology, Guangzhou City 510006, Guangdong Province, China.
Gene. 2014 May 15;541(2):107-14. doi: 10.1016/j.gene.2014.03.011. Epub 2014 Mar 11.
Aspergillus niger is usually regarded as a beneficial species widely used in biotechnological industry. Obtaining the genome sequence of the widely used aconidial A. niger SH2 strain is of great importance to understand its unusual production capability. In this study we assembled a high-quality genome sequence of A. niger SH2 with approximately 11,517 ORFs. Relatively high proportion of genes enriched for protein expression related FunCat items verify its efficient capacity in protein production. Furthermore, genome-wide comparative analysis between A. niger SH2 and CBS513.88 reveals insights into unique properties of A. niger SH2. A. niger SH2 lacks the gene related with the initiation of asexual sporulation (PrpA), leading to its distinct aconidial phenotype. Frame shift mutations and non-synonymous SNPs in genes of cell wall integrity signaling, β-1,3-glucan synthesis and chitin synthesis influence its cell wall development which is important for its hyphal fragmentation during industrial high-efficiency protein production.
黑曲霉通常被认为是一种有益的物种，广泛应用于生物技术产业。获得广泛使用的分生孢子型黑曲霉 SH2 菌株的基因组序列对于了解其异常的生产能力非常重要。在本研究中，我们组装了一个高质量的黑曲霉 SH2 基因组序列，大约有 11517 个开放阅读框。相对较高比例的与蛋白质表达相关的 FunCat 项目富集的基因证实了其在蛋白质生产方面的高效能力。此外，黑曲霉 SH2 与 CBS513.88 之间的全基因组比较分析揭示了黑曲霉 SH2 独特性质的见解。黑曲霉 SH2 缺乏与无性孢子形成（PrpA）起始相关的基因，导致其具有明显的分生孢子表型。细胞壁完整性信号、β-1,3-葡聚糖合成和几丁质合成基因中的移码突变和非同义 SNP 影响其细胞壁发育，这对于其在工业高效蛋白质生产过程中的菌丝片段化非常重要。
